Cefotetan

Manufactured by Merck Group

Sourced in United States, Sao Tome and Principe

Cefotetan is a cephalosporin antibiotic used in the treatment of various bacterial infections. It functions as a broad-spectrum antimicrobial agent by inhibiting cell wall synthesis in susceptible bacteria.

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2 protocols using cefotetan

Cefotetan Cytotoxicity and MAPK Signaling

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Cefotetan was purchased from Sigma-Aldrich (St Louis, USA). All cell culture media and supplements were from HyClone (Logan, USA).
¹⁵N-ammonium chloride was purchased from Cambridge Isotope Laboratories. The phospho-p44/42 MAPK (p-ERK) antibody, p44/42 MAPK (ERK) antibody, HRP-labelled goat anti-rabbit IgG and HRP-labelled goat anti-mouse IgG secondary antibodies were from Beyotime (Shanghai, China). RKIP antibody and β-actin antibody were purchased from Cell Signaling Technology (Danvers, USA) and Proteintech (Wuhan, China), respectively. The transfection agent lipotap was obtained from Beyotime. A CellTiter 96
^® Aqueous kit was purchased from Promega (Madison, USA).

Guo C., Xu H., Zhou Y., Wu Z., Jiang B., Chen H, & Lin D. (2022). Cefotetan-bound human RKIP involves in Ras/Raf1/MEK/ERK signaling pathway: Structural basis of the cefotetan-hRKIP interaction. Acta Biochimica et Biophysica Sinica, 54(12), 1917-1923.

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Salmonella Serotyping and Antimicrobial Susceptibility

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Salmonella isolates were serotyped via the slide agglutination assay according to the instructions provided by the manufacturer of the Salmonella antisera (Statens Serum Institute, Copenhagen, Denmark). The serovars of the isolates were determined using the Kauffmann-White scheme (Grimont and Weill, 2007 ).
Antimicrobial susceptibility testing of Salmonella isolates was performed using the agar dilution method for ampicillin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, cefuroxime, cefotetan, ceftazidime, ceftriaxone, cefepime, aztreonam, imipenem, amikacin, gentamicin, and tobramycin (Sigma, St. Louis, MO, United States). Escherichia coli ATCC 25922 was used as a quality control strain in each run. The breakpoints for determining susceptibility or resistance to antimicrobials were determined by the guidelines of the Clinical Laboratory Standard Institute (CLSI, 2018 ).

Lyu N., Feng Y., Pan Y., Huang H., Liu Y., Xue C., Zhu B, & Hu Y. (2021). Genomic Characterization of Salmonella enterica Isolates From Retail Meat in Beijing, China. Frontiers in Microbiology, 12, 636332.

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