Homozygous CaMKIIα-Cre line T29–1 transgenic mice (Jackson Laboratory #005359) were crossed with homozygous Ai32 mice (Jackson Laboratory #012569) to express channelrhodopsin2 (ChR2) in neurons expressing male and female CaMKIIα in F1 hybrid mice (N = 5; 3 male; 25–40g, 30–50 weeks of age). After implantation, animals were housed individually on a reversed 12/12 h day/night schedule. Following one week of recovery, mice were recorded 5–7 days/week for two months before being euthanized with pentobarbital cocktail (Euthasol®, transcardial 300 mg/kg) and perfused with formalin (10%). All experiments were conducted in accordance with the Institutional Animal Care and Use Committee of New York University Medical Center.
T29 1 transgenic mice
Manufactured by Jackson ImmunoResearch
T29–1 transgenic mice are a laboratory animal model developed for research purposes. They are genetically modified to express specific genes or traits of interest to researchers. The core function of these mice is to serve as a tool for scientific investigation, without any interpretation or extrapolation on intended use.
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3 protocols using t29 1 transgenic mice
1
CaMKIIα-Cre Mice for Channelrhodopsin2 Expression
2
Intracellular Recordings in Anesthetized Rodents
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All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at New York University Medical Center and the Ethics Committee of the Instituto Cajal (CSIC). Intracellular recordings in anesthetized rats were obtained in Madrid and were performed according to the Spanish legislation (R.D. 1201/2005 and L.32/2007), the European Communities Council Directives of 1986 (86/609/EEC) and 2003 (2003/65/CE) for animal research. All animals were kept in the vivarium on a 12-h light/dark cycle and were housed 2–3 per cage. Following surgery, the mice were moved to a 12-hour reverse light cycle (lights on/off at 7 pm/am) and housed individually. Prior to behavior training, mice were provided food and water ad libitum, but were water restricted to maintain 85% of their weight during and after behavioral training. Homozygous CaMKIIa-Cre line T29–1 transgenic mice (Jackson Laboratory #005359) were crossed with homozygous Ai32 mice (Jackson Laboratory #012569) to express channelrhodopsin2 (ChR2) in neurons expressing CaMKIIa in F1 hybrid mice. We used n = 7 male F1 hybrid mice (n = 3 mice for extracellular recordings and n = 4 for intracellular recordings in head-fixed conditions; 25–40 g, 30–50 weeks of age) and n = 10 male and female Wistar rats (240–450 gr; 20 to 50 weeks of age).
3
Intracellular Recordings in Anesthetized Rodents
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