The anti-phospho-MAPKs (JNK, p38MAPK, and ERK) and anti-phospho-IκB antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-GAPDH antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Nigericin was purchased from Tocris (Bristol, UK). Eucalyptol, ATP, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Anti phospho iκb antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho-IκB antibodies are immunological reagents that detect phosphorylated forms of the IκB protein. IκB is a key regulator of the NF-κB signaling pathway, which is involved in various cellular processes. These antibodies can be used to study the activation and regulation of the NF-κB pathway in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Eucalyptol, by Merck Group (1 mentions)
Nigericin, by Bio-Techne (1 mentions)
β actin monoclonal antibody, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti phospho akt, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti c jun n terminal kinase jnk, by Cell Signaling Technology (1 mentions)
Human rankl, by Thermo Fisher Scientific (1 mentions)
Anti phospho gsk3β, by Cell Signaling Technology (1 mentions)
Anti extracellular signal regulated kinase erk, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
2 protocols using anti phospho iκb antibodies
1
Antibody Validation for MAPK Signaling
2
Osteoclastogenesis Regulation Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Reagents Soluble, recombinant human macrophage colony-stimulating faotor (M-CSF) and human RANKL were obtained from PeproTech EC, Ltd. (London, U.K.). Lipopolysaccharide (LPS) from Escherichia (E.) coli was reconstituted in sterilized water to give a stock concentration of 10 mg/ mL and stored at -20°C until use. Anti-p38, anti-phospho-p38, anti-c-Jun N-terminal kinase (JNK), anti-phospho-JNK, anti-Akt, anti-phospho-Akt, anti-glycogen synthase kinase 3β (GSK3β), anti-phospho-GSK3β, anti-extracellular signalregulated kinase (ERK), anti-phospho-ERK, anti-inhibitor (I)κB, and anti-phospho-IκB antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, U.S.A.). Antic-Fos and anti-NFATc1 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). A monoclonal β-actin antibody was obtained from Sigma (St. Louis, MO, U.S.A.). Fetal bovine serum (FBS), α-minimum essential medium (α-MEM), and penicillin/streptomycin were purchased from Gibco BRL (Grand Island, NY, U.S.A.). All other chemicals were of analytical grade or complied with the standards required for cell culture.
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