Tp53 do 7

Manufactured by Agilent Technologies

Sourced in United States

The TP53 (DO-7) is a lab equipment product designed for the detection and analysis of the TP53 protein. TP53 is a tumor suppressor gene that plays a crucial role in regulating cell growth and division. The product provides a tool for researchers to study the expression and function of TP53 in various biological systems.

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Lab products found in correlation

Ab195477, by Abcam (1 mentions) Mef2d, by BD (1 mentions) H3k27me3, by Abcam (1 mentions) Ab4729, by Abcam (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Rack1, by Santa Cruz Biotechnology (1 mentions) Hdac4, by Abcam (1 mentions) Lamin b1, by Abcam (1 mentions) H3k27ac, by Abcam (1 mentions) Ab16048, by Abcam (1 mentions) Benchmark xt system, by Roche (1 mentions) Ema e29, by Agilent Technologies (1 mentions) P16 clone jc8, by Santa Cruz Biotechnology (1 mentions) Ck7 ov tl, by BioGenex (1 mentions) Ck5 clone xm26, by Zytomed Systems (1 mentions) Ck19 rck108, by Agilent Technologies (1 mentions) Anti nut antibody clone c52b1, by Cell Signaling Technology (1 mentions)

2 protocols using tp53 do 7

Antibody Detection Assay for Epigenetic Markers

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Antibodies used were against MEF2D (BD Bioscience), H3K27ac (ab4729; Abcam, Cambridge, MA, USA). H3K27me3 (ab195477, Abcam) HDAC4 (Paroni et al., 2004), γH2AX (9718, Cell Signalling, Leiden, The Netherlands), TP53 (DO‐7; Dako, Santa Clara, CA, USA), LT SV40 (sc‐147, Santa Cruz), Lamin B1 (ab16048, Abcam), p21 (CP74, Sigma), RACK1 (sc‐17754, Santa Cruz, Dallas, TX, USA), GFP (Paroni et al., 2004).

Paluvai H., Di Giorgio E, & Brancolini C. (2018). Unscheduled HDAC4 repressive activity in human fibroblasts triggers TP53‐dependent senescence and favors cell transformation. Molecular Oncology, 12(12), 2165-2181.

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Comprehensive Immunohistochemical Profiling of Tissue Samples

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The tissue specimen was fixed in formalin and processed routinely for histopathology. Immunohistochemistry (IHC) was performed on 3-µm sections cut from paraffin blocks using a fully automated system (“Benchmark XT System”, Ventana Medical Systems Inc, 1910 Innovation Park Drive, Tucson, Arizona, USA) and the following antibodies: CK7 (OV-TL, 1:1000, Biogenex), p63 (SSI6, 1: 100, DCS), CK5 (clone XM26, 1: 50, Zytomed), EMA (E29, 1:20, Dako), CEA (polyclonal, 1:400, Dako), CK19 (RCK108, 1:300, Dako), p16 (clone JC8, 1:100, Santa Cruz Biotechnology), TP53 (DO-7, 1:50, Dako) and anti-NUT antibody (clone C52B1, 1:45, Cell Signaling).

Agaimy A., Stoehr R., Tögel L., Hartmann A, & Cramer T. (2020). YAP1-MAML2-Rearranged Poroid Squamous Cell Carcinoma (Squamoid Porocarcinoma) Presenting as a Primary Parotid Gland Tumor. Head and Neck Pathology, 15(1), 361-367.

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