Primary antibodies included rat anti-GFAP (Invitrogen, CA); α-tubulin (Sigma-Aldrich, St. Louis, MO, USA), Estrogen Receptor α (C1355) (Millipore, Temecula, CA), Estrogen Receptor β, clone 68-4 (Millipore), S100A4 (Abcam), Pan-cytokeratin MNF116 (Dakocytomation, Denmark); Phospho-EGF Receptor (Tyr1068) and total EGFR (Cell Signaling, Boston, MA). For western blot, secondary antibodies were Alexa-fluor 680 Anti-Rabbit or Anti-Mouse IgG (Invitrogen, NY) detected using an Odyssey Infrared Imaging System (Licor Biosciences, Lincoln, NE). For immunohistochemistry, secondary antibodies were anti-mouse Alexafluor-488 or anti-rabbit Alexafluo-555 (Invitrogen). ER antagonists included 4-OH-tamoxifen (Sigma-Aldrich) and ICI 182780 (Tocris bioscience; R&D systems, Minneapolis, MN). The EGFR/HER2 inhibitor Lapatinib was purchased from Selleckchem (Houston, TX).
Alexa fluor 680 anti rabbit or anti mouse igg
Manufactured by Thermo Fisher Scientific
Alexa Fluor 680 conjugated antibodies are fluorescent labeling reagents designed for use in immunofluorescence, flow cytometry, and other fluorescence-based applications. These antibodies specifically bind to rabbit or mouse IgG, and the Alexa Fluor 680 dye provides a bright, photostable fluorescent signal in the far-red region of the spectrum.
Automatically generated - may contain errors
Lab products found in correlation
4 oh tamoxifen, by Merck Group (2 mentions)
Anti mouse alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
α tubulin, by Merck Group (2 mentions)
Rat anti gfap, by Thermo Fisher Scientific (2 mentions)
Ici 182 780, by Bio-Techne (2 mentions)
Odyssey infrared imaging system, by LI COR (2 mentions)
Lapatinib, by Selleck Chemicals (2 mentions)
Phospho egf receptor tyr1068, by Cell Signaling Technology (2 mentions)
Total egfr, by Cell Signaling Technology (2 mentions)
Estrogen receptor α c1355, by Merck Group (2 mentions)
Pan cytokeratin mnf116, by Agilent Technologies (2 mentions)
Anti rabbit alexafluo 555, by Thermo Fisher Scientific (2 mentions)
2 protocols using alexa fluor 680 anti rabbit or anti mouse igg
1
Breast Cancer Molecular Profiling Protocol
2
Breast Cancer Molecular Profiling Protocol
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Primary antibodies included rat anti-GFAP (Invitrogen, CA); α-tubulin (Sigma-Aldrich, St. Louis, MO, USA), Estrogen Receptor α (C1355) (Millipore, Temecula, CA), Estrogen Receptor β, clone 68-4 (Millipore), S100A4 (Abcam), Pan-cytokeratin MNF116 (Dakocytomation, Denmark); Phospho-EGF Receptor (Tyr1068) and total EGFR (Cell Signaling, Boston, MA). For western blot, secondary antibodies were Alexa-fluor 680 Anti-Rabbit or Anti-Mouse IgG (Invitrogen, NY) detected using an Odyssey Infrared Imaging System (Licor Biosciences, Lincoln, NE). For immunohistochemistry, secondary antibodies were anti-mouse Alexafluor-488 or anti-rabbit Alexafluo-555 (Invitrogen). ER antagonists included 4-OH-tamoxifen (Sigma-Aldrich) and ICI 182780 (Tocris bioscience; R&D systems, Minneapolis, MN). The EGFR/HER2 inhibitor Lapatinib was purchased from Selleckchem (Houston, TX).
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