Anti-TRIM44 polyclonal antibody (Proteintech Group, 11,511-1-AP); anti-Ub antibody (Biolegend, 646,301); anti-mCherry antibody (ThermoFisher, PA5-34,974); anti-VIM antibody (ThermoFisher, MA5-11,883); anti-NFE2L2/NRF2 antibody (ThermoFisher, PA5-27,882); anti-20S proteasome antibody (MilliporeSigma, ST1049); anti-TUBG/γ-Tubulin antibody (MilliporeSigma, T5326); anti-ATG5 antibody (Novus Biologicals, NBP2-24,389); Anti-GFP antibody (Santa Cruz Biotechnology, sc-9996); anti-ACTB/β-Actin antibody (Santa Cruz Biotechnology, sc-47,778), anti-HA antibody (Santa Cruz Biotechnology, sc-805); anti-BECN1 antibody (Cell Signaling Technology, 4122); anti-HDAC6 antibody (Cell Signaling Technology, 7558); anti-LC3B antibody (Cell Signaling Technology, 3868); anti-SQSTM1/p62 antibody (Cell Signaling Technology, 88,588); anti-cleaved PARP antibody (Cell Signaling Technology, 5625); Alexa Fluor 594 donkey anti rabbit IgG(H + L) (Invitrogen, A21207); Alexa Fluor 546 goat anti mouse IgG(H + L) (Invitrogen, A11003).
Anti sqstm1 p62 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-SQSTM1/p62 antibody is a laboratory reagent used to detect the SQSTM1/p62 protein. SQSTM1/p62 is a multifunctional protein involved in various cellular processes. This antibody can be used to study the expression and localization of SQSTM1/p62 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti lc3b antibody, by Cell Signaling Technology (2 mentions)
Anti gfp antibody, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved parp antibody, by Cell Signaling Technology (1 mentions)
Anti hdac6 antibody, by Cell Signaling Technology (1 mentions)
Anti trim44 polyclonal antibody, by Proteintech (1 mentions)
Anti ub antibody, by BioLegend (1 mentions)
Anti mcherry antibody, by Thermo Fisher Scientific (1 mentions)
Anti atg5 antibody, by Novus Biologicals (1 mentions)
Alexa fluor 594 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 546 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Anti ha antibody, by Santa Cruz Biotechnology (1 mentions)
Precast polyacrylamide gel, by Bio-Rad (1 mentions)
Enhanced chemiluminescence, by Cytiva (1 mentions)
Anti mtor antibody, by Cell Signaling Technology (1 mentions)
Anti α synuclein antibody, by BD (1 mentions)
Anti bip antibody, by GeneTex (1 mentions)
Anti chop antibody, by Cell Signaling Technology (1 mentions)
Anti atg5 antibody, by Cell Signaling Technology (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti lamp1, by Cell Signaling Technology (1 mentions)
4 protocols using anti sqstm1 p62 antibody
1
Protein Expression and Antibody Validation
2
α-Synuclein Detection and Quantification
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Proteins were separated on precast polyacrylamide gels (Bio-Rad) and transferred to Hybond (Amersham). For detection of α-synuclein, proteins were cross-linked to membranes using 0.4% paraformaldehyde (51 , 52 ). Chemiluminescent detection was performed with enhanced chemiluminescence (Amersham). Antibodies used were anti-α-synuclein antibody ([1:1500], BD Transduction Laboratories, 610787), anti-Ataxin-2 antibody ([1:4000], BD Biosciences, 611378), anti-Staufen antibody ([1:5000], Novus Biologicals, NBP1-33202), anti-BiP antibody ([1:3000], Genetex, GTX113340), anti-CHOP antibody ([1:3000], Cell Signaling Technology, 2895), anti-mTOR antibody ([1:4000], Cell Signaling Technology, 2972), anti-SQSTM1/p62 antibody ([1:4000], Cell Signaling Technology, 5114), anti-LC3B antibody ([1:7000], Novus Biologicals, NB100-2220SS), anti-tyrosine hydroxylase antibody ([1:2000], Millipore-Sigma, AB152), and peroxidase-conjugated monoclonal anti-β-Actin antibody (clone AC-15) ([1:20,000], Sigma-Aldrich, A3854). The secondary antibody was peroxidase-conjugated anti-rabbit (Jackson ImmunoResearch Laboratories).
3
Immunohistochemical Analysis of p62 in Mouse Liver
4
Plasmid Construction for Autophagy Study
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The pcDNA EGFP-RFP-LC3B plasmid encoding EGFP, RFP, and LC3B was constructed by the pcDNA 3.1 myc-his (-) vector. The pcDNA EGFP-mRFP-LC3B (ptfLC3) plasmid encoding Chlorocebus sabaeus LC3B (NCBI Reference Sequence: XM_007994295.2) was constructed by the pcDNA 3.1 myc-his (-) vector. Anti-LC3B antibody (Cell Signaling, Danvers, MA, USA, #2775), anti-SQSTM1/p62 antibody (Cell Signaling, #5114), anti-ATG5 antibody (Cell Signaling, #9980), anti-ATG12 antibody (Cell signaling, #4180), anti-LAMP1 (Cell Signaling, #3243), anti-β-actin (Santa Cruz, sc-47778), and mouse anti-PEDV antibody were made in our laboratory (immunized inactivated PEDV in mouse).
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