Apoptotic detection kit

Manufactured by Roche

Sourced in Switzerland

The Apoptotic detection kit is a laboratory tool used to detect and analyze apoptosis, a form of programmed cell death. The kit provides the necessary reagents and protocols to perform assays that measure various markers associated with the apoptotic process, allowing researchers to study cell death mechanisms.

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Lab products found in correlation

Dm il led inverted phase contrast microscope, by Leica (1 mentions) Fluorescence microscopy, by Olympus (1 mentions)

Spelling variants of this product

TUNEL apoptotic cell detection kit (6 citations) Apoptotic kit (3 citations) In situ apoptotic cell death detection kit (3 citations) In situ apoptotic cell detection kit (2 citations) Apoptotic cell detection kit (2 citations) In situ apoptotic detection kit (2 citations)

5 protocols using apoptotic detection kit

Evaluating Toxicity and Apoptosis of 131I-Au PENPs-CTX

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To investigate the potential toxicity of the ¹³¹I-Au PENPs-CTX in vivo, one mouse from each group treated with ¹³¹I-Au PENPs-CTX, ¹³¹I-Au PENPs, Au PENPs-CTX, Au PENPs, or saline was euthanized to extract the tumor and major organs (heart, liver, spleen, lung, and kidneys) after 21 days of treatment. The excised tumors and organs were fixed, embedded, sectioned, and stained with hematoxylin and eosin (H&E), and then observed using an AMEX 1200 inverted phase contrast microscope (Thermo Fisher Scientific, Inc.). Furthermore, tumor apoptosis was analyzed using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method and an apoptotic detection kit (Roche, Basel, Switzerland). The TUNEL kit was used for fixation, dehydration, paraffin-embedding, sectioning, and staining; then the tumor sections were observed with a Leica DM IL LED inverted phase contrast microscope.

Zhao L., Li Y., Zhu J., Sun N., Song N., Xing Y., Huang H, & Zhao J. (2019). Chlorotoxin peptide-functionalized polyethylenimine-entrapped gold nanoparticles for glioma SPECT/CT imaging and radionuclide therapy. Journal of Nanobiotechnology, 17, 30.

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Apoptosis Assessment in Tumor Tissue

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After the imaging experiments, one mouse from each group was sacrificed and the tumors and major organs were extracted. According to the standard procedure of hematoxylin and eosin (H&E) staining, the tumors and organs were fixed, embedded, sectioned, stained, and observed. The tumor tissue apoptosis of mice after DOX treatment was further confirmed using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method by apoptotic detection kit (Roche, Basel, Switzerland) according to the literature (Zhao et al., 2015 (link)). Through the treatments of fixation, dehydration, paraffin-embedment, sectioning, and staining using a TUNEL kit, the tumor sections were finally observed.

Xing Y., Zhu J., Zhao L., Xiong Z., Li Y., Wu S., Chand G., Shi X, & Zhao J. (2018). SPECT/CT imaging of chemotherapy-induced tumor apoptosis using 99mTc-labeled dendrimer-entrapped gold nanoparticles. Drug Delivery, 25(1), 1384-1393.

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TUNEL Assay for Apoptosis Detection

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Apoptosis of tissues and cells was measured by TUNEL assay. Apoptotic detection kit (Roche, Switzerland) was used to investigate DNA fragments in the nucleus in situ according to the manufacturer's instructions. Fluorescence microscopy was used to capture images. TUNEL-positive cells and total cells were counted, and the ratio was calculated.

You L., Zheng Y., Yang J., Hou Q., Wang L., Zhang Y., Zhao C, & Xie R. (2022). LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling. Mediators of Inflammation, 2022, 5463505.

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Apoptosis Detection by TUNEL Assay

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Apoptosis of tissue and cell was assessed by TUNEL assay. Apoptotic detection kit (Roche) was used to detect DNA fragments in the nucleus in situ. TUNEL‐positive cells and total cells were counted, while the ratio was calculated.

Ren B., Zhang Y., Liu S., Cheng X., Yang X., Cui X., Zhao X., Zhao H., Hao M., Li M., Tie Y., Qu L, & Li X. (2020). Curcumin alleviates oxidative stress and inhibits apoptosis in diabetic cardiomyopathy via Sirt1‐Foxo1 and PI3K‐Akt signalling pathways. Journal of Cellular and Molecular Medicine, 24(21), 12355-12367.

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Apoptosis Quantification via TUNEL Assay

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After CHIP plasmid transfection and challenged with HG, cells were fixed with 4% Paraformaldehyde for 1 h at RT. After washing with PBS, cells were permeabilized with Triton X‐100 (0.1%) in sodium citrate (0.1%), and incubated with TUNEL reagent to measure apoptosis using apoptotic detection kit (Roche Diagnostic). In cardiac tissue, slides were deparaffinized, rehydrated followed by incubation with 3% H₂O₂. Thereafter, sections were washed, and incubated with TUNEL reagent for 1 h at 37°C. Next, cells were incubated with DAPI for 5 min, followed by washing with PBS. Finally, apoptosis was examined by detecting the TUNEL‐positive cells using fluorescence microscopy (Olympus) having excitation and emission wavelength of 450–500 nm and 515–565 nm, respectively. The number of TUNEL‐positive cells counted manually and statistically analyzed using GraphPad Prism5 software.

Ali A., Kuo W., Kuo C., Lo J., Chen M.Y., Daddam J.R., Ho T., Viswanadha V.P., Shibu M.A, & Huang C. (2021). E3 ligase activity of Carboxyl terminus of Hsc70 interacting protein (CHIP) in Wharton's jelly derived mesenchymal stem cells improves their persistence under hyperglycemic stress and promotes the prophylactic effects against diabetic cardiac damages. Bioengineering & Translational Medicine, 6(3), e10234.

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