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Ab93915

Manufactured by Abcam
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Ab93915 is a laboratory product distributed by Abcam. It is a tool used for scientific research purposes. No further details are available.

Automatically generated - may contain errors

Lab products found in correlation

Ab8295, by Abcam (2 mentions) Ab97023, by Abcam (2 mentions) Ab201228, by Abcam (2 mentions) Ab205718, by Abcam (2 mentions) Clarity western ecl chemiluminescent substrate, by Bio-Rad (2 mentions) Sc 398608, by Proteintech (2 mentions) Ab6046, by Abcam (1 mentions) Ab6046, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ab93915

1

Optimized Protein Immunoblotting Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were loaded and resolved under denaturing conditions on a NuPAGE 3% to 8% Tris-Acetate gel or NuPAGE 4% to 20% Tris-Glycine gel. Lysate was transferred to a PVDF membrane overnight at 40 mA at 4°C. Skim milk at 5% in 1× TBST buffer was used as a blocking agent. Primary antibodies used for Western blotting include mouse anti-FLAG 1:1000 (Sigma-Aldrich F1804), rabbit anti-HA 1:1000 (CST C29F4), rabbit anti-MYOM1 1:1000 (Abcam ab201228), mouse anti-cardiac troponin T 1:500 (Abcam ab8295), rabbit anti-MYOM2 1:500 (Abcam ab93915), rabbit anti-TUBB 1:1000 (Abcam ab6046), mouse anti-MuRF1 (muscle RING-finger protein 1) 1:1000 (Santa Cruz sc-398608), and rabbit anti-CAPN3 (calpain 3) 1:1000 (Proteintech 104-92-1-AP). Blots were blocked for at least 30 minutes at room temperature before addition to primary antibodies, incubated at 4°C overnight, and washed 3× with TBST before addition of mouse or rabbit secondary antibodies (Abcam ab97023, ab205718). Secondary antibodies were applied for 1 to 2 hours at 1:5000, washed 3× with TBST, and detected using Clarity Western ECL chemiluminescent substrates per the manufacturer’s protocol (Bio-Rad 1705060).
Agarwal R., Wakimoto H., Paulo J.A., Zhang Q., Reichart D., Toepfer C., Sharma A., Tai A.C., Lun M., Gorham J., DePalma S.R., Gygi S.P., Seidman J.G, & Seidman C.E. (2022). Pathogenesis of Cardiomyopathy Caused by Variants in ALPK3, an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere. Circulation, 146(22), 1674-1693.
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2

Western Blot Analysis of Muscle Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were loaded and resolved under denaturing conditions on a NuPAGE 3-8% Tris-Acetate gel or NuPAGE 4-20% Tris-Glycine gel. Lysate was transferred to a PVDF membrane overnight at 40mA at 4°C. 5% skim milk in 1X TBST buffer was used as a blocking agent. Primary antibodies used for Western blotting include mouse anti-FLAG 1:1000 (Sigma-Aldrich, F1804), rabbit anti-HA 1:1000 (CST, C29F4), rabbit anti-MYOM1 1:1000 (Abcam, ab201228), mouse anti-cardiac troponin T 1:500 (Abcam, ab8295), rabbit anti-MYOM2 1:500 (Abcam, ab93915), rabbit anti-TUBB 1:1000 (Abcam, ab6046), mouse anti-MuRF1 1:1000 (Santa Cruz, sc-398608), rabbit anti-CAPN3 1:1000 (Proteintech, 104-92-1-AP). Blots were blocked for at least 30 minutes at room temperature prior to addition to primary antibodies, incubated at 4°C overnight, washed 3X with TBST prior to addition of mouse or rabbit secondary antibodies (Abcam ab97023, ab205718). Secondary antibodies were applied for 1-2 hours at 1:5000, washed 3X with TBST, and detected using Clarity Western ECL chemiluminescent substrates per manufacturer protocol (Bio-Rad, 1705060).
Agarwal R., Wakimoto H., Paulo J.A., Zhang Q., Reichart D., Toepfer C., Sharma A., Tai A.C., Lun M., Gorham J., DePalma S.R., Gygi S.P., Seidman J.G, & Seidman C.E. (2022). Pathogenesis of Cardiomyopathy Caused by Variants in ALPK3, an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere. Circulation, 146(22), 1674-1693.
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