Cisplatin and curcumin were purchased from Sigma, the cell counting kit‐8 was purchased from DOjinDO (CCK‐8, DOjinDO Laboratories), and the miRNeasy mini‐kit was purchased from Qiagen. MiR‐192 mimics and miR‐192 inhibitor were obtained from GenePharma. Lipofectamine 2000 transfection reagent was purchased from Life technologies. Monoclonal antibody specific to NKRF, p‐κB, IκB, NF‐κB p65, PARP‐1, cIAP1, cIAP2, Bcl‐xl and XIAP were purchased from Abcam, cell signaling technology (Danvers) and Santa Cruz, respectively. pNFκB‐luc was purchased from Beyotime, pMIR‐GLO dual‐luciferase miRNA target report vector and dual‐luciferase reporter assay system were purchased from Promega.
Mir 192 mimics
Manufactured by GenePharma
Sourced in China
The MiR-192 mimics is a laboratory product designed to mimic the function of the microRNA miR-192. MicroRNAs are small non-coding RNA molecules that play a role in the regulation of gene expression. The MiR-192 mimics can be used in research applications to study the effects of this specific microRNA.
Automatically generated - may contain errors
Lab products found in correlation
Mir 192 inhibitor, by GenePharma (2 mentions)
Nf κb p65, by Abcam (1 mentions)
Curcumin, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Parp1, by Abcam (1 mentions)
Ciap2, by Abcam (1 mentions)
Bcl xl, by Abcam (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Mirneasy mini kit, by Qiagen (1 mentions)
Ciap1, by Abcam (1 mentions)
Pnfκb luc, by Beyotime (1 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Lipofectamine reagent, by Thermo Fisher Scientific (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using mir 192 mimics
1
Cisplatin and Curcumin Modulate NF-κB Signaling
2
Oxidative Stress and miRNA in GCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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GCs, collected from healthy follicles (diameter: 3–5 mm), were cultured at 37 °C in T25 flasks containing DMEM/F-12 medium (Gibco, Grand Island, NY, USA) 10% FBS, and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) in 5% CO2. For drug administration, GCs were treated with 0 to 1000 μM H2O2 for 12 h. The following miRNAs were transfected GCs: miR-192 mimics, miR-192 inhibitor, negative control (NC) mimics, and inhibitor NC (Shanghai Gene Pharma, Shanghai, China); this was achieved using Lipofectamine reagent (Invitrogen, Shanghai, China) according to the manufacturer’s protocol.
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