Wild-type (WT) pEGFP-C2-WWC3, pEGFP-C2-WWC3-ΔWW, and the corresponding pEGFP-C2 empty vectors were gifts from Dr Joachim Kremerskothen (University of Münster, Germany). pGPU6-shNC-RFP, pGPU6-shWWC3-1-RFP, and pGPU6-shWWC3-2-RFP were purchased from GenePharma (Shanghai, China). LATS1 siRNA (sc-35797), YAP siRNA (sc-38637), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Stable clonal cell lines were selected with 1,800 μg/mL of G418 (Thermo Fisher Scientific, Waltham, MA, USA) and were maintained at 900 μg/mL of G418. Lipofectamine 3000 (Thermo Fisher Scientific) transfection reagent was used for plasmid transfection.
Yap sirna sc 38637
Manufactured by Santa Cruz Biotechnology
Sourced in United States
YAP siRNA (sc-38637) is a pool of 3 target-specific 19-25 nt siRNA designed to knock down the expression of the YAP (Yes Associated Protein) gene in human cells.
Automatically generated - may contain errors
2 protocols using yap sirna sc 38637
1
Plasmid Transfection and Cell Line Generation
2
Investigating YAP, Cyr61, and Apoptosis Signaling
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The primary antibodies, anti-YAP (sc-15407) was purchased from Santa-cruz (USA), anti-Cyr61 (11952S), Bcl-2(#2870S) and BAX (#2772) were from Cell signaling technology (CST, USA), and anti-βactin (BS6007M) was purchased from Bioworld (USA). The secondary antibodies, horseradish peroxidaseconjugated goat anti-rabbit IgG (BS13278) and horseradish peroxidase-conjugated goat anti-mouse IgG (#7076S) were purchased from CST (USA), and horseradish peroxidase-conjugated goat anti-rabbit IgG (BS13278) was purchased from Bioworld (USA). The secondary antibody, fluorophore-labeled goat antirabbit IgG (ab350077) was purchased from abcam (UK). PTX and VP used in chemotherapeutic drug experiments were purchased from Sigma (USA). YAP-siRNA (sc-38637) was purchased from Santa-cruz (USA). The plasmids used in transfection were purchased from Genloci Biotechnoligies Inc. (China). Control experiments were performed using one set of cells, and then repeated at least three times.
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