N 3 tert butoxycarbonyl aminopropyl methacrylamide apma boc

Chemicals such as 1-amino-propan-2-ol, 2,2′-azobisisobutyronitrile (AIBN), methacryloyl chloride, 6-amino hexanoic acid, tert-butoxycarbonyl hydrazide, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), carbon disulfide, ethanethiol, sodium hydride (60% dispersion in mineral oil), 5-hydroxy-2-pentanone, 4,6-trinitrobenzene-1-sulfonic acid (TNBSA), pentafluorophenol, 4-(dimethylamino)pyridine (DMAP), tert-butanol, N,N-dimethylacetamide (DMA), dichloromethane (DCM), dimethyl sulfoxide (DMSO), and 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy, free radical (4-oxo-TEMP) were obtained from Merck (Czech Republic). Pyropheophorbide-a was purchased from Frontier Scientific^® (USA), 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) from Wako Chemicals (Germany), and N-(3-tert-butoxycarbonyl-aminopropyl)methacrylamide (APMA-Boc) from Polysciences, Inc., (USA). All of the other chemicals and solvents were of analytical grade. The solvents for the nuclear magnetic resonance (NMR) characterization of DMSO-d₆ (99.80 atom% D) and CD₃OD (99.80 atom% D) were obtained from VWR Chemicals (Belgium).

1,4-Dioxane, 2,2′-azobisisobutyronitrile (AIBN), 2-cyanopropan-2-yl dithiobenzoate (CTA-AIBN), 2-thiazoline-2-thiol, 2,4,6-trinitrobenzene-1-sulfonic acid (TNBSA), 3-azido-1-propylamine, 4,4′-azobis(4-cyanopentanoic acid) (ACVA), 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTA-ACVA), dimethyl sulfoxide (DMSO), methacryloyl chloride, N,N-diisopropylethylamine (DIPEA), N,N-dimethylacetamide (DMA), phosphate buffered saline (phosphate buffer 0.01M and NaCl 0.154M, pH 7.4) (PBS), and t-butanol were purchased from Sigma-Aldrich (Prague, Czech Republic). 2,2′-Azobis(4-methoxy-2,4-dimethylvaleronitrile) (V-70) was from Fujifilm Wako Chemicals Europe (Neuss, Germany). 3-Amino-1-(11,12-didehydrodibenzo[b,f]azocin-5(6H)-yl)propan-1-one (DBCO-NH₂) was from Click Chemistry Tools (Scottsdale, AZ, USA). N-(3-tert-butoxycarbonyl-aminopropyl)methacrylamide (APMA-Boc) was purchased from Polysciences, Inc. (Warrington, PA, USA), and 1-aminopropan-2-ol was from TCI Europe (Zwijndrecht, Belgium). All solvents and chemicals were of analytical grade.