To confirm which metal autometallography was demonstrating, since autometallography can also detect inorganic silver and bismuth [29 (link), 30 (link)], and to look for the presence of other potentially toxic elements, 7 μm paraffin sections of representative sections from patient PD2 were deparaffinised and subjected to LA-ICP-MS for mercury, silver, bismuth, aluminium, gold, cadmium, chromium, iron, nickel, lead and potassium. Analyses were carried out on a New Wave Research NWR-193 laser and a Teledyne Cetac LSX-213 G2+ laser hyphenated to an Agilent Technologies 7700x ICP-MS, with argon used as the carrier gas. LA-ICP-MS conditions were optimised on NIST 612 Trace Element in Glass CRM and the sample was ablated with a 50 μm spot size and a scan speed of 100 μm/s at a frequency of 20 Hz. The data were collated into a single image file using in-house developed software [31 (link)] and visualised using FIJI.
Lsx 213 g2 laser
Manufactured by Agilent Technologies
Sourced in United States
The LSX-213 G2+ laser is a solid-state laser system designed for laser ablation applications. It provides a focused laser beam with wavelength of 213 nanometers, pulse duration of 4-7 nanoseconds, and repetition rates up to 20 Hertz. The laser can be used for various analytical techniques that require laser ablation, such as elemental analysis and depth profiling.
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Lab products found in correlation
4 protocols using lsx 213 g2 laser
1
Elemental Analysis of Parkinsonian Tissue
2
Multielement Imaging of Kidney Samples
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To confirm which metal autometallography was demonstrating, since autometallography can also detect inorganic silver and bismuth [47 (link),48 (link)], and to look for the presence of other toxic metals, 7 μm paraffin sections of six kidney samples were deparaffinised and subjected to LA-ICP-MS for mercury, silver, bismuth, aluminium, gold, cadmium, chromium, iron, nickel, and lead. Analyses were carried out on a New Wave Research NWR-193 laser or a Teledyne Cetac LSX-213 G2+ laser hyphenated to an Agilent Technologies 7700x ICP-MS, with argon used as the carrier gas. LA-ICP-MS conditions were optimised on NIST 612 Trace Element in Glass CRM and the sample was ablated with a 50 µm spot size and a scan speed of 100 µm/s at a frequency of 20 Hz. The data were collated into a single image file using in-house developed software and visualised using FIJI.
3
Toxic Metal Profiling in Thyroid Tissue
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To confirm which metal autometallography was demonstrating (since autometallography can also detect inorganic silver and bismuth) and to look for the presence of other toxic metals, 7 μm paraffin sections of selected thyroid samples were deparaffinised and subjected to LA-ICP-MS for mercury, silver, bismuth, aluminium, gold, cadmium, chromium, iron, nickel and lead, as well as for phosphorus (contained in cell nuclei) to assess cellular density. Analyses were carried out on a New Wave Research NWR-193 laser or a Teledyne Cetac LSX-213 G2+ laser hyphenated to an Agilent Technologies 7700x ICP-MS, with argon used as the carrier gas. LA-ICP-MS conditions were optimised on NIST 612 Trace Element in Glass CRM and the sample was ablated with a 50 μm spot size and a scan speed of 100 μm/s at a frequency of 20 Hz. The data were collated into a single image file using in-house developed software and visualised using FIJI.
4
Identifying Metals in Pancreas Tissue
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To confirm which metal autometallography was demonstrating (since autometallography can also detect inorganic silver and bismuth), 7 μm paraffin sections of selected pancreas samples were deparaffinised and subjected to LA-ICP-MS for mercury, silver, bismuth, aluminium, gold, cadmium, chromium, iron, nickel and lead, as well as for zinc to localise islets due to their high zinc levels [20 (link)]. Analyses were carried out on a Teledyne Cetac LSX-213 G2+ laser (Omaha, NE, USA) hyphenated to an Agilent Technologies 8900 ICP-MS (Santa Clara, CL, USA), with argon used as the carrier gas. LA-ICP-MS conditions were optimised on NIST 612 Trace Element in Glass CRM (US Department of Commerce, Gaithersburg, MD, USA) and the sample was ablated with a 50 µm spot size and a scan speed of 100 µm/s at a frequency of 20 Hz. The data were collated into a single image file using in-house developed software and visualised using FIJI open source image processing (LOCI, University of Wisconsin, WI, USA).
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