In the current study, various solvents with different polarities were used to prepare the various extracts, and the extraction process was carried out, with minor modifications in agreement with El Ghouizi et al.,11 . Shortly, 10 mL of each selected solvent (ethyl acetate, chloroform, hexane, hydroethanolic 70% v/v, acetone, and distilled water) were added to 1 g of plant powder, and the mixture was macerated and sustained under continuous agitation at room temperature for one week. After a previously performed test, which examined a variety of solvents, these conditions showed the most promising results (data not shown). Whatman N°1 filter was used to remove impurities from the extracts and thereafter condensed using a rotary vacuum evaporator at 40 °C. The resulting crude extracts were collected and kept at −20 °C before use.
N 1 filter
Manufactured by Cytiva
The N°1 filter is a laboratory filtration device designed to separate solid particles from liquids or gases. It features a high-quality filter medium that effectively removes contaminants, ensuring the purity of the filtered material. The N°1 filter is a reliable and versatile tool for various applications in research and industrial settings.
Automatically generated - may contain errors
3 protocols using n 1 filter
1
Optimization of Plant Extract Preparation
2
Nuclear Genome Size Estimation of Phytophthora Species
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nuclear genome size of selected isolates of P. xcambivora, P. xheterohybrida and P. xincrassata was assessed using laser flow cytometry. Phytophthora isolates were grown in clarified V8-broth for three to seven days at 20 °C. The mycelium was washed thoroughly with deionized water and blotted dry on a Whatman N°1 filter. Nuclei were prepared and stained using the CyStain PI Absolute P kit (Sysmex Partec GmbH, Görlitz, Germany). About 1 mg mycelium was chopped simultaneously with 0.5 cm2 of young Raphanus sativus cv Saxa leaf tissue in 500 μL extraction buffer using a razor blade. The samples were filtered through a 10 μm CellTrics® filter to remove cellular debris. Nuclei were stained using 2 mL of staining solution and incubated overnight at 4 °C. Stained nuclei were analysed using a Partec PAS III flow cytometer (Sysmex Partec GmbH, Görlitz, Germany) equipped with a 488 nm Argon laser. Histograms were rendered and analysed using Flomax software (Sysmex Partec GmbH, Görlitz, Germany). The genome size of each sample was calculated by multiplying the genome size of the internal standard (Raphanus; 1086 Mbp) with the ratio of the fluorescence peaks of the sample and the internal standard. Nuclei of all isolates were measured on two days, each time in triplicate.
3
Microwave-Assisted Extraction of Phenolic Compounds from Bellis perennis Flowers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extraction of phenolic compounds from B. perennis flowers was performed using a microwave system (MAXMOS23S, Maxi power model, 2450MHZ, China). An aliquot of flower powder was placed in a 250 mL round flask containing the appropriate extraction solvent according to the experimental design in which the influence of each parameter was studied. The parameters evaluated were ethanol concentration (40-80%), irradiation time (60-120s), microwave power (200-400W), and solvent to solid ratio (40-80 mL/g). After irradiation, the extracts were recovered by filtration through a Whatman N°1 filter. Determination of total phenolic content TPC was determined according to the method of Taga et al. (1984) . Briefly, a volume of 100 μL of each extract was mixed with 2.0 mL of 2% Na2CO3, after 4min, 100 µL of 50% Folin-Ciocalteu reagent was added, and this mixture was incubated in the dark at room temperature for 30 min. The absorbance was measured at 750 nm using a UV/VIS spectrophotometer (Shimadzu UV spectrophotometer). The experiment was performed in triplicate, the polyphenol contents were calculated from a calibration curve performed with gallic acid, and the results were expressed in milligrams of gallic acid equivalent per gram of sample dry weight (mg GAE/g dw).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!