Clock lab analysis 6

Analysis of circadian parameters of core body temperature, total
locomotion, and wheel running activity was measured using Clock Lab Analysis 6
(Actimetrics, Wilmette, IL, USA). Period, phase angle of entrainment (difference
between onset of activity/peak in core body temperature and onset of dark
phase), phase, and amplitude of wheel running rhythms and total loco motor
activity as measured by emitters were analyzed for all tumor treatment groups
during 14:10 LD and DD light cycles. Daily percentages of activity during the
light and dark phases of 14:10 LD were computed for total loco motor and wheel
running activities. The period and phase of peak body temperature was also
analyzed in 14:10 LD and DD. The seventh day of the 14:10 LD period (the day
prior to DD transition) was removed from the analysis due to technical software
recording errors.

Electronic telemetry devices (G2 E-mitters, Starr Life Sciences, Oakmont, PA) were surgically implanted into the peritoneal cavity using an aseptic technique as previously described [35 (link)]. Mice recovered from surgery with in-cage running wheels (Starr-Life Sciences) with a 120.7 mm diameter and 50.8 mm width for 1 week prior to data collection. Following this recovery period, cages were placed on receiving platforms (ER4000, Starr Life Sciences) to continuously record core body temperature and total locomotion. Glass probes recorded voluntary wheel running at 1-min sampling intervals using VitalView 5.1 software (Starr Life Sciences). ClockLab Analysis 6 (Actimetrics, Wilmette, IL, USA) generated 24-h graphs of wheel running, locomotion, and body temperature. All wheel running, locomotion, and body temperature graphs were double-plotted to better visualize trends in chemotherapy-induced fatigue (average dark phase running wheel revolutions and locomotor emitter counts) and fever (°C). In Experiment 2, a recording error prevented data acquisition on days 25-27 (11-13 d following chemotherapy) but did not affect statistical differences in recovery from fatigue measures. VitalView software was used to ensure that voluntary wheel running occurred when analyzing effects of exercise on body composition and central and peripheral inflammation.