Rat anti l1 cam

Manufactured by Merck Group

Rat anti-L1-CAM is a laboratory reagent used to detect and study the L1-CAM (L1 cell adhesion molecule) protein in various biological samples. L1-CAM is a cell surface glycoprotein involved in cell-cell adhesion and neuronal development. The rat anti-L1-CAM antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and analyze the expression of L1-CAM in different cell types and tissues.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Low melting point agarose, by Promega (1 mentions) Hoechst 33342 nuclear stain, by Thermo Fisher Scientific (1 mentions) Cy3 conjugated donkey anti rat igg, by Jackson ImmunoResearch (1 mentions) Vt1000p vibratome, by Leica (1 mentions) Goat anti chat, by Merck Group (1 mentions) Alexa 488 donkey anti goat igg, by Thermo Fisher Scientific (1 mentions) Goat anti brn3a, by Santa Cruz Biotechnology (1 mentions) Mouse anti vinculin, by Merck Group (1 mentions) Sp5 confocal microscope, by Zeiss (1 mentions) Rabbit anti s100β, by Agilent Technologies (1 mentions) Rabbit anti fibronectin, by Merck Group (1 mentions) Rat anti cd31, by BD (1 mentions) Rabbit anti olig2, by Merck Group (1 mentions) Chicken anti myelin protein zero p0, by Abcam (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Rabbit anti ki67, by Abcam (1 mentions) Rabbit anti iba1, by Fujifilm (1 mentions)

3 protocols using rat anti l1 cam

Immunohistochemical Staining of Brain Sections

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Immunohistochemical staining of free floating brain sections was performed on 70 μm coronal brain sections obtained from fixed brains embedded in a 1.5% (w/v) mix of 0.75% Low-Melting Point Agarose (Promega) and 0.75% standard Agarose (Fisher) and cut on a Leica VT 1000P vibratome. Sections were immersed in blocking solution (5% normal donkey serum, 1% BSA, 0.2% glycine, 0.2% lysine with 0.3% TritonX-100 in PBS) and incubated on a rotating shaker for 1 hour at room temperature. Sections were next incubated overnight at 4°C with rat anti-L1-CAM, 1:200 (Millipore, MAB5272MI) diluted in blocking solution. Sections were washed three times with PBS before fluorescent secondary antibody incubation with Cy3-conjugated donkey anti-rat IgG (Jackson). Sections were counterstained with 50μg/ml Hoechst 33342 nuclear stain (Life Tech. #H21492) diluted in 1xPBS for 5 minutes at room temperature and serially mounted onto glass slides with Aquamount.

Robichaux M.A., Chenaux G., Ho H.Y., Soskis M.J., Greenberg M.E., Henkemeyer M, & Cowan C.W. (2015). EphB1 and EphB2 Intracellular Domains Regulate the Formation of the Corpus Callosum and Anterior Commissure. Developmental neurobiology, 76(4), 405-420.

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Antibody Labeling for Immunofluorescence and Western Blotting

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The following antibodies were used in this study: rabbit anti‐MYO9B (#12432‐1‐AP, Proteintech), mouse antivinculin (#05‐386, Millipore), rat anti‐myelin basic protein (MBP; #MAB386, Millipore), rat anti‐L1‐CAM (#MAB5272, Millipore), chicken anti‐NF‐M (#822701, Biolegend), goat anti‐ChAT (#AB144P, Millipore), and goat anti‐Brn3a (SC:8429, Santa Cruz Biotechnology).
For immunofluorescence, secondary antibodies included fluorescein (FITC)‐ and rhodamine (TRITC)‐conjugated donkey anti‐rat, chicken, or rabbit IgG (Jackson ImmunoResearch), and Alexa‐488 donkey anti‐Goat IgG (#A11055, Invitrogen).
For Western blotting, secondary antibodies included horseradish peroxidase‐conjugated goat antirabbit (#P0448, DAKO) and rabbit antimouse (#P0260, DAKO) immunoglobulins.

Cipriani S., Guerrero‐Valero M., Tozza S., Zhao E., Vollmer V., Beijer D., Danzi M., Rivellini C., Lazarevic D., Pipitone G.B., Grosz B.R., Lamperti C., Marzoli S.B., Carrera P., Devoto M., Pisciotta C., Pareyson D., Kennerson M., Previtali S.C., Zuchner S., Scherer S.S., Manganelli F., Bähler M, & Bolino A. (2022). Mutations in MYO9B are associated with Charcot–Marie–Tooth disease type 2 neuropathies and isolated optic atrophy. European Journal of Neurology, 30(2), 511-526.

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Immunostaining of Brain Tissue Sections

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Frozen sections of 12 μm thickness were subject to a standard protocol for immunofluorescence staining as described previously (Zhao et al., 2008 (link)). Where required, heat-mediated antigen retrieval was performed using a commercial antigen retrieval solution (Sigma-Aldrich). The following antibodies were used: goat /rabbit anti-GFP (Abcam), rabbit anti-Olig2 (Millipore), rabbit anti-GFAP (Dako), rabbit anti-periaxin (gift from Professor Peter Brophy or from Sigma-Aldrich), rabbit anti-S100β (Dako), rat anti-PDGFRa (CD140a; BD Bioscience), rabbit anti-prolyl-4 hydroxylase β (P4HB; Abcam), rabbit anti-HSP47 (BioVision), rabbit anti-IBA1 (Wako), rabbit anti-smooth muscle actin (SMA; Abcam), rabbit anti-Ki67 (Abcam), chicken anti-myelin protein zero (P0) (Abcam), goat anti-Sox2 and goat anti-Sox10 (Santa Cruz Biotechnology), rat anti-CD31 (BD Biosciences), rabbit anti-fibronectin (Millipore), rat anti-L1cam (Millipore), and rabbit anti-Foxj1 (Insight Biotechnology) Secondary antibodies against relevant primary antibodies labeled with either Alexa Fluor 488 or Alexa Fluor 594 were from Thermo Fisher Scientific. The images were acquired with a Leica SP5 confocal microscope or a Zeiss Axio Observer A1 fluorescence Imaging System.

Ma D., Wang B., Zawadzka M., Gonzalez G., Wu Z., Yu B., Rawlins E.L., Franklin R.J, & Zhao C. (2018). A Subpopulation of Foxj1-Expressing, Nonmyelinating Schwann Cells of the Peripheral Nervous System Contribute to Schwann Cell Remyelination in the Central Nervous System. The Journal of Neuroscience, 38(43), 9228-9239.

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