Irisin recombinant elisa kit

The enzyme-linked immunosorbent assay (ELISA) tests were performed on serum samples from BC patients. Serum samples were obtained from venous blood after centrifugation at 3000 rpm for 10 min and were preserved at −80 °C for further analysis. Serum Ir levels were examined using commercially available ELISA recombinant irisin kit test Irisin, Recombinant Elisa kit (Cat No. EK-0670-29, Phoenix Pharmaceuticals, CA, USA). The ELISA tests were performed according to the manufacturer’s protocol. For each sample, 50 μL of serum was used. The results were verified by microplate reader ELX-800 (BIO-TEK, VT, USA). The examination was performed in two repetitions.

Fasting glucose, insulin, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides serum levels were measured in all children studied. The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated (insulin (μU/mL) × glucose (mmol/L)/22.5) [45 (link)].
Irisin concentrations were determined in plasma using the irisin recombinant ELISA kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA). The intra-assay and inter-assay coefficients of variation (CVs) were ≤10% and ≤15%, respectively; sensitivity was 2.42 ng/mL and specificity was 100% for human irisin (1–112) and 9% for FNDC5 isoform 4.

Plasma levels of METRNL and irisin were measured using the Enzyme Linked Immunosorbent Assay (ELISA). Plasma samples were thawed on ice and centrifuged for 5 min at 10,000 ×g at 4°C to remove any remaining cells or platelets. Plasma level of METRNL was measured using the Human METRNL ELISA kit (LifeSpan BioSciences, Inc., Seattle, WA, USA. Cat# LS-F13315). The samples were diluted 10× with sample diluent. ELISA was performed according to the manufacturer’s instructions. The intra-assay coefficient of variation was 5.0%–10.0% while the inter-assay coefficient of variation was < 10%. Plasma level of irisin was measured using the irisin recombinant ELISA kit (Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA. Cat #EK-067-29) following the manufacturer’s kit instructions. Plasma samples were diluted 40× with the 1× assay buffer (provided in the kit). The intra-assay coefficient for this ELISA assay was 1.0%–7.0%, while the inter-assay coefficient was < 20%.