Anti klf1

Manufactured by Abcam

Sourced in United States

Anti-KLF1 is a primary antibody that recognizes the Krüppel-like factor 1 (KLF1) protein. KLF1 is a transcription factor that plays a key role in the regulation of erythroid cell development and hemoglobin production.

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Lab products found in correlation

Anti n cadherin, by Abcam (1 mentions) Anti bcl 2, by Abcam (1 mentions) Anti β catenin, by Abcam (1 mentions) Anti β actin, by Abcam (1 mentions) Anti e cadherin, by Abcam (1 mentions) Ripa lysis buffer, by Aspen (1 mentions) Facsaria 2 sorp, by BD (1 mentions) Anti actin, by Merck Group (1 mentions)

2 protocols using anti klf1

Protein Expression Analysis in A549 Cells

Cited 1 time

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Total proteins from A549 cells were isolated using RIPA lysis buffer (Aspen Biotechnology, Wuhan, China). BCA kit was performed to quantify the concentration of proteins. After that, proteins (10 μg/lane) were separated by 10% SDS-PAGE and then transferred onto PVDF membranes. Next, PVDF membranes were incubated with primary antibodies anti-KLF1 (Abcam, Cambridge, MA, USA; 1:1000; Rabbit), anti-E-cadherin (1:1000; Rabbit), anti-N-cadherin (1:1000; Rabbit), anti-β-catenin (1:1000; Rabbit), anti-cleaved caspase 3 (1:1000; Rabbit), anti-Bcl-2 (1:1000; Rabbit), or anti-β-actin (1:1000; Rabbit) at 4°C overnight. Subsequently, PVDF membranes were incubated with the secondary antibodies (1:5000; Goat Anti-Rabbit) for 1 h. Then, the protein bands were observed using an efficient chemiluminescence (ECL) kit according to the previous literature [35 (link)]. β-actin was viewed as an internal control.

Chen Y., Hong C., Qu J., Chen J, & Qin Z. (2022). Knockdown of lncRNA PCAT6 suppresses the growth of non-small cell lung cancer cells by inhibiting macrophages M2 polarization via miR-326/KLF1 axis. Bioengineered, 13(5), 12834-12846.

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KLF1 Protein Expression Analysis

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We adopted a previously described WB procedure (Green and Sambrook, 2012 ). Leukocytes/WBCs of Klf1(K74R) mice and WT B6 mice, respectively, were collected from RBC lysis buffer-treated PB. CD3⁺ T cells, B220⁺ B cells, and NK cells from the spleen were sorted using FACS AriaII SORP (BD Bioscience). The cell pellets were lysed in sample buffer and run on SDS-PAGE gels. WB with anti-KLF1 (Abcam) and anti-actin (Sigma) antibodies was then used to analyze and compare the levels of KLF1 and actin proteins.

Wang J.P., Hung C.H., Liou Y.H., Liu C.C., Yeh K.H., Wang K.Y., Lai Z.S., Chatterjee B., Hsu T.C., Lee T.L., Shyu Y.C., Hsiao P.W., Chen L.Y., Chuang T.J., Yu C.H., Liao N.S, & Shen C.K. (2024). Long-term hematopoietic transfer of the anti-cancer and lifespan-extending capabilities of a genetically engineered blood system by transplantation of bone marrow mononuclear cells. eLife, 12, RP88275.

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