Male Sprague-Dawley rats (weight, 180–200 g; age, 6–8 weeks; n=32) were purchased from the Experimental Animal Center of Wenzhou Medical University (Wenzhou, China). Rats were housed under a controlled temperature (22–25°C), humidity (40–60%) and light environment (12-h dark/light), and fed with standard rat chow (10–15 g twice a day) and water (20–45 ml a day), and this access was controlled, except for one day of fasting prior to the operation. The weight-matched rats were randomly assigned to one of four groups: Sham-operated, treated with vehicle (saline, n=8) or SSBE (100 mg/kg/day, n=8), and unilateral ureteral obstruction (UUO) treated with vehicle (n=8) or SSBE (100 mg/kg/day, n=8). UUO surgery was performed as previously described (11 (link)). All rats were sacrificed by cervical dislocation and were anesthetized by 0.2% pentobarbital natrium (Sigma-Aldrich; Merck Millipore, Darmstadt, Germany). Kidneys were excised at day 8 for rats in the UUO SSBE and vehicle control groups as previously described (11 (link)). SSBE (cat. no. 20101017; Xuancheng Baicao Plant Industry and Trade Co., Ltd., Anhui, China) was extracted according to the standard protocol (10 (link)). The animal study protocols were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University.
Pentobarbital natrium
Manufactured by Merck Group
Sourced in Germany
Pentobarbital natrium is a barbiturate compound commonly used as a sedative and hypnotic agent in laboratory settings. It serves as a central nervous system depressant and is primarily utilized for animal research and euthanasia procedures. The product specification and core function are to provide a controlled and consistent means of achieving sedation or euthanasia in laboratory environments.
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Lab products found in correlation
Sybr green master mix, by Toyobo (1 mentions)
Hematoxylin and eosin he, by Boster Bio (1 mentions)
Chemiluminescent hrp substrate, by Merck Group (1 mentions)
3 3 di aminobenzidine, by Boster Bio (1 mentions)
Optimal cutting temperature compound o c t, by Sakura Finetek (1 mentions)
Embryomax m2 medium, by Merck Group (1 mentions)
Embryomax ksom, by Merck Group (1 mentions)
Embryomax htf, by Merck Group (1 mentions)
Revertra ace qpcr rt master mix with gdna remover kit, by Toyobo (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Oil red o, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Hyaluronidase, by Merck Group (1 mentions)
2 protocols using pentobarbital natrium
1
Sprague-Dawley Rat UUO Model Study
2
Comprehensive Cell Death Evaluation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TRIzol reagent was purchased from Invitrogen. ReverTra Ace qPCR RT Master Mix with gDNA Remover Kit and SYBR Green Master Mix were obtained from TOYOBO (Osaka, Japan). Hyaluronidase, Oil Red O, pentobarbital natrium and paraformaldehyde were purchased from Sigma-Aldrich. 3,3′-diaminobenzidine and hematoxylin and eosin (HE) were obtained from Bosterbio. Optimal cutting temperature compound (OCT) was purchased from Sakura Finetek (Torrance, CA, USA). Pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) were obtained from Ningbo Secondary Hormone Corp. (Ningbo, China) . In Situ Cell Death Detection Kit (Fluorescein) was purchased from Roche Applied Sciences. Chemiluminescent HRP Substrate, Embryo max HTF, EmbryoMax M2 medium, Embryo Max KSOM were obtained from Millipore. Oil for embryo culture was purchased from Irvine Science (Santa Ana, CA, USA). Halt Protease & Phosphatase Inhibitor Cocktail was obtained from Pierce. Bicinchoninic acid (BCA) protein assay kit and RIPA lysis and extraction buffer were purchased from Dingguo (Beijing, China). The details, suppliers and dilution of antibodies used in this study are reported in Table 1. All other chemicals were of reagent grade and were obtained from standard commercial sources.
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