Rabbit anti p mad

Manufactured by Abcam

Sourced in United States, Japan

Rabbit anti-p-mad is an antibody product that recognizes the phosphorylated form of the Mothers against decapentaplegic homolog (Mad) protein. Mad is a key transcription factor in the TGF-beta signaling pathway. This antibody can be used to detect the activated, phosphorylated state of Mad in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Lsm880 laser, by Zeiss (1 mentions) Plan apochromat 63x 1.4 oil dic m27, by Zeiss (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Anti p smad3, by Abcam (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Goat anti gfp, by Abcam (1 mentions) Fluorescein isothiocyanate (fitc), by Jackson ImmunoResearch (1 mentions) Mouse anti β galactosidase, by Promega (1 mentions) Mouse anti hts, by Developmental Studies Hybridoma Bank (1 mentions) Rat anti vasa, by Developmental Studies Hybridoma Bank (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Rat anti ha, by Roche (1 mentions)

Close spelling variants of this product

Rabbit anti-

3 protocols using rabbit anti p mad

Visualizing Indirect Flight Muscle Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To observe the individual indirect flight muscles (IFMs), heads and abdomens were removed and the remaining body was fixed in 4% paraformaldehyde (PFA) for 1 h at room temperature (RT) or overnight at 4 °C. Then, the muscular tissue was obtained from the dissected thorax in 0.3% PBST and blocked for 1 h in 0.3% PBST with 10% normal goat serum for 1 h at RT. Primary antibodies were rabbit anti-cleaved caspase 3 (1:200, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-p-mad (a gift from Prof. Peter ten Dijke) and anti-p-smad3 (1:200, Abcam, Cambridge, UK), secondary antibodies conjugated to DyLight 488 or 649 (Jackson Immuno Research, West Grove, PA, USA) were used at 1:200. The muscle fibers were labeled with rhodamine-phalloidin (1:1000, Thermo Fisher Scientific, Bedford, MA, USA) and nuclei were counterstained with DAPI (1 µg/mL; Sigma Aldrich, Saint Louis, MI, USA). The samples were observed with Zeiss LSM880 laser scanning confocal microscopy, with a Plan-Apochromat 63x/1.4 Oil DIC M27 objective.

Deng J., Guan X.X., Zhu Y.B., Deng H.T., Li G.X., Guo Y.C., Jin P., Duan R.H, & Huang W. (2022). Reducing the Excess Activin Signaling Rescues Muscle Degeneration in Myotonic Dystrophy Type 2 Drosophila Model. Journal of Personalized Medicine, 12(3), 385.

+ Open protocol

+ Expand

Larval Tracheal Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Larval tracheae were dissected at either L2 or L3 larval instar and immunostained according to standard protocols. The following primary antibodies were used: mouse anti-Hdc (1:1), mouse anti-β-galactosidase 40.1a (1:200) from the Hybridoma Bank, rabbit anti-PH3 (Ser) (1:100) from Cell signaling, rabbit anti pMad (1:100) a gift from Gines Morata and goat anti-GFP (1:500) from Abcam. Secondary antibodies labeled with Alexa 488, Alexa 555, or Alexa683 were obtained from Molecular Probes. Micrographs were acquired with Leica SP5 and SPE confocal microscopes and images were processed with FIJI.

Djabrayan N.J, & Casanova J. (2016). Snoo and Dpp Act as Spatial and Temporal Regulators Respectively of Adult Progenitor Cells in the Drosophila Trachea. PLoS Genetics, 12(3), e1005909.

+ Open protocol

+ Expand

Immuno-detection of Drosophila Ovary Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adult ovaries were dissected, fixed, and stained according to Eliazer et al. (2011) (link), except that ovaries stained for pMAD were fixed for 30 minutes. The images were taken with Zeiss LSM 510 confocal microscope. The following primary antibodies were used: mouse anti-Hts (1B1 from Developmental Studies Hybridoma Bank, dilution at 1:20), rat anti-VASA (Developmental Studies Hybridoma Bank, DSHB, 1:20), mouse anti-Lamin C (LC28.26, DSHB, 1:20), anti-Engrailed (4D9, DSHB, 1:2), mouse anti-Sxl (M114 DSHB, 1:10), rabbit anti-pMAD (Abcam, 1:200) mouse anti-BamC A7 (gift from D. McKearin, 1:20), rabbit anti-Nanos (gift from A. Nakamura, RIKEN, Kobe, Japan), rabbit anti-Spectrin (gift from R. Dubreuil, University of Illinois at Chicago, 1:2,500), mouse anti-β-galactosidase (Promega, 1:1,000), rabbit anti-GFP (Invitrogen, 1:1,000), guinea pig anti-Traffic Jam (gift from D. Godt, University of Toronto, Toronto, ON, Canada, 1:5,000), rat anti-HA (Roche, 3F10). Cy3, Cy5, FITC (Jackson Laboratories) or Alexa 488 (Molecular Probes) fluorescence-conjugated secondary antibodies were used at a 1:200 dilution.

Mottier-Pavie V.I., Palacios V., Eliazer S., Scoggin S, & Buszczak M. (2016). The Wnt pathway limits BMP signaling outside of the germline stem cell niche in Drosophila ovaries. Developmental biology, 417(1), 50-62.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!