Plasmid DNA listed in Table S2 was stored and propagated in Top10 Escherichia coli (InvitrogenTM). For plasmid isolation, E. coli were grown in 2YT media (w/v: 1.6% tryptone, 1% yeast extract, 0.5% NaCl) containing either 100 µg/mL ampicillin sodium salt (Melford Ipswich, UK) or 50 µg/mL kanamycin monosulphate (Formedium, Hunstanton, UK) each diluted from 1000× frozen stock.
Ampicillin sodium salt
Manufactured by Formedium
Sourced in United Kingdom
Ampicillin sodium salt is a commonly used antibiotic compound that belongs to the penicillin class of medications. It functions as an antimicrobial agent, inhibiting the growth and replication of certain bacteria. This product is primarily used in laboratory settings for research and experimental purposes.
Automatically generated - may contain errors
Lab products found in correlation
Escherichia coli top10, by Thermo Fisher Scientific (1 mentions)
Ethylenediaminetetraacetic acid disodium salt dihydrate, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
N butanol, by Merck Group (1 mentions)
Chloramphenicol, by Carl Roth (1 mentions)
1 4 dithiothreitol, by Carl Roth (1 mentions)
Hepes, by Carl Roth (1 mentions)
2 protocols using ampicillin sodium salt
1
Plasmid DNA Storage and Propagation
2
Purification of Membrane Protein Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All chemicals and media were as
reported previously,10 (link) with the following
additions: 1,4-dithiothreitol (Carl Roth); ampicillin sodium salt
(Formedium); 14C-l -arginine (stock solution 325
mCi/mmol; NovaTec); 2-mercaptoethanol (Sigma-Aldrich); chloramphenicol
(Carl Roth); coenzyme A, free acid (Avanti Polar Lipids);d -(+)-fructose 1,6-bisphosphate trisodium salt hydrate (Sigma-Aldrich); d (+)-glucose anhydrous (Formedium); d (+)-sucrose (Formedium);
ethylenediaminetetraacetic acid disodium salt dihydrate (Sigma-Aldrich);
HEPES (Carl Roth); methanol (Sigma); n-butanol (Sigma); n-dodecyl-β-d -maltopyranoside, Anagrade (Anatrace);
oleic acid (Sigma-Aldrich); tris hydrochloride (ITW Reagents); and
TritonTM X-100 (Sigma-Aldrich).
reported previously,10 (link) with the following
additions: 1,4-dithiothreitol (Carl Roth); ampicillin sodium salt
(Formedium); 14C-
mCi/mmol; NovaTec); 2-mercaptoethanol (Sigma-Aldrich); chloramphenicol
(Carl Roth); coenzyme A, free acid (Avanti Polar Lipids);
ethylenediaminetetraacetic acid disodium salt dihydrate (Sigma-Aldrich);
HEPES (Carl Roth); methanol (Sigma); n-butanol (Sigma); n-dodecyl-β-
oleic acid (Sigma-Aldrich); tris hydrochloride (ITW Reagents); and
TritonTM X-100 (Sigma-Aldrich).
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