IL-10 levels in the serum and in the cell-free supernatants were determined by the corresponding cytokine ELISA kits (eBioscience, San Diego, CA, USA), according to the corresponding manufacturer’s instructions. Red blood cell (RBC)-depleted splenocytes were cultured at 1×106 cells per well with or without KCs in a total volume of 200 µL at a ratio of 2:1 (splenocytes: KCs). Splenocytes were stimulated with anti-CD3 (1 µg/mL) and anti-CD28 (1 µg/mL) (BD Bioscience, Franklin Lakes, NJ, USA). Interferon (IFN)-γ levels in the cell-free supernatants were determined by the corresponding cytokine enzyme-linked immunosorbent assay (ELISA) kits (eBioscience), according to the corresponding manufacturer’s instructions.
Cytokine enzyme linked immunosorbent assay elisa kits
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cytokine enzyme-linked immunosorbent assay (ELISA) kits are laboratory tools used to quantify the levels of specific cytokines in biological samples. These kits employ the ELISA technique, which is a sensitive and reliable method for detecting and measuring the concentration of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Cytokine elisa kit, by Thermo Fisher Scientific (1 mentions)
Anti cd3, by BD (1 mentions)
Anti cd28, by BD (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Monocyte chemotacticprotein 1 mcp 1, by Thermo Fisher Scientific (1 mentions)
Activin a, by R&D Systems (1 mentions)
Rpmi 1640 medium, by Procell (1 mentions)
Huv ec c, by American Type Culture Collection (1 mentions)
Guava cell cycle reagent, by Merck Group (1 mentions)
Annexin 5 kit, by Thermo Fisher Scientific (1 mentions)
Hel299, by American Type Culture Collection (1 mentions)
4 protocols using cytokine enzyme linked immunosorbent assay elisa kits
1
Cytokine ELISA for IL-10 and IFN-γ
2
Activin A Regulation of Inflammatory Response
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Activin A was obtained from R&D Systems, Inc. Fetal bovine serum (FBS) was purchased from Biological Industries Israel Beit-Haemek Ltd. Cytokine enzyme linked immunosorbent assay (ELISA) kits were provided by eBioscience (San Diego, USA). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich (Merck KGaA). A one-step reverse transcription-polymerase chain reaction (RT-PCR) kit was provided by Takara Biotechnology Co., Ltd. Monocyte chemotactic protein 1 (MCP-1) was purchased from PeproTech.
3
H9N2 Avian Influenza Virus Vaccine Protocol
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The A/Chicken/Jiangsu/JS-1/2002 (H9N2) AIV was isolated and maintained in our laboratory [31 (link)]. Oil-formulated inactivated H9N2 AIV vaccine was purchased from Qian Yuan Hao (China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG was obtained from Boshide Corporation (China), and HRP-conjugated goat anti-mouse IgG1 and anti-mouse IgG2a were obtained from Bethyl Corporation (Germany). Fluorescein isothiocyanate-labeled anti-mouse CD3 and phycoerythrin-labeled anti-mouse CD4, CD8 antibodies were obtained from Caltag (China). Enzyme-linked immunosorbent assay (ELISA) cytokine kits were purchased from eBioscience (USA). RPMI 1640 medium was purchased from Procell (China). The four BPs (I–IV) were synthesized by Shanghai Science Peptide Biological Technology (China), and their purities were over 95%.
4
Comprehensive Cell Line Analysis
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All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise indicated. A549, HEL299, and HUV-EC-C [human umbilical cord endothelial cells (HUVEC)] cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Annexin-V kit, anti-CD133-fluorescein isothiocyanate (FITC) antibody, and enzyme-linked immunosorbent assay (ELISA) cytokine kits were obtained from e-Bioscience (San Diego, CA, USA). Recombinant human hepatocyte growth factor (HGF), transendothelial migration assay kit, and Guava cell cycle reagent were obtained from Millipore Corp. (Boston, MA, USA). All antibodies for Western blotting, unless specified, were purchased from Abcam (Cambridge, UK). Caspases 3 and 9, Bcl-2, Bax, and actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-nuclear factor κB (NF-κB) p65 (S529) phycoerythrin (PE) antibodies were from Thermo Scientific (Waltham, MA, USA).
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