The following materials were obtained from Sigma-Aldrich (St. Louis, MO, USA): 5-HT, N-(3-dimethylaminopropyl)-Nʹ-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS), lactoperoxidase (LPO). Fe3O4@PEG-COOH was purchased from Nanjing Nanoeast Biotech Co., Ltd. (Nanjing, China). Vivotrax and Perimag were purchased from Magnetic Insight Inc (Alameda, USA) and Micromod Partikeltechnologie GmbH (Rostock, Germany), respectively. Cy7-NHS was obtained from AAT Bioquest (Sunnyvale, CA, USA). RAW 264.7 cells (Otwo Biotech Inc., Shenzhen, China), Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich), fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), and cell counting kit-8 (CCK-8; Beyotime Institute of Biotechnology, Shanghai, China) were obtained for cell experiments. Myeloperoxidase (Abcam, Cambridge, UK), glucose oxidase (Dalian Meilun Biotechnology Co., Ltd., Dalian, China), Matrigel (Corning, Inc., Corning, NY, USA), 4-ABAH (Sigma-Aldrich), and ExiTron (TM) nano 12000 CT contrast agent (Miltenyi Biotec, Bergisch Gladbach, Germany) were purchased for animal experiments. Erythropoietin protein (EPO), rabbit anti-mouse MPO, rabbit anti-mouse CD68, rabbit anti-mouse anti-ɑ-smooth muscle actin (SMA), rabbit anti-mouse CD31, and rabbit anti-mouse MCP-1 antibodies were obtained from Abcam.
Cy7 nhs
Manufactured by AAT Bioquest
Sourced in United States, China
Cy7-NHS is a succinimidyl ester derivative of the cyanine dye Cy7. It is a fluorescent labeling reagent used for the covalent attachment of Cy7 dyes to biomolecules, such as proteins, peptides, and nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
Myeloperoxidase, by Abcam (1 mentions)
Rabbit anti mouse cd68, by Abcam (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions)
4 abah, by Merck Group (1 mentions)
Rabbit anti mouse cd31, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Matrigel, by Corning (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Uv 3600, by Shimadzu (1 mentions)
F 7000, by Hitachi (1 mentions)
Cck 8 assay, by Solarbio (1 mentions)
2 protocols using cy7 nhs
1
Multimodal Imaging Probe Characterization
2
Cy7-labeled Nerve-binding Peptide Characterization
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Nerve-binding peptide (NP41, China Peptides Co. Ltd.) was labeled with Cy7 monosuccinimidyl ester (Cy7-NHS; AAT Bioquest Inc.) at 25°C as in a previous study (46 (link)). Briefly, Cy7-NHS and NP41 were dissolved in dimethyl sulfoxide (Aladdin), respectively. After overnight shaking in darkness, the product was dialyzed for 24 hours to remove unconjugated Cy7-NHS or NP41. Last, the product was lyophilized to obtain Cy7-NP41. The fluorescence intensity of Cy7-NP41 was measured at various concentrations (0, 0.3, 0.6, 1.25, 2.5, 5, 10, 20, 40, 80, and 159 μM). The steady conjugation of Cy7-NHS and NP41 was characterized by mass spectroscopy. Both optical absorption spectra of the Cy7-NP41 and Cy7-NHS were acquired by a UV-vis spectrophotometer (UV3600, Shimadzu, Japan). Fluorescence excitation and emission spectrum were monitored using a fluorescence spectrofluorometer (F-7000, Hitachi, Japan). The optical absorption peaks of Cy7-NP41 and Cy7 dissolved in fetal bovine serum (FBS; Gibco) were monitored at different time points over 48 hours. All reactions were performed in the dark. The cytotoxicity of the Cy7-NP41 was evaluated by Cell Counting Kit-8 (CCK-8 assay, Solarbio).
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