Left ventricular myocardial tissues were collected and snap-frozen in liquid nitrogen and stored in a freezer at −80°C. Malondialdehyde (MDA) content (E-EL-0060c, Elabscience Biotechnology Co., Ltd), ROS content (E-BC-K138-F, Elabscience Biotechnology Co., Ltd), and SOD activity (E-BC-K019-S, Elabscience Biotechnology Co., Ltd) were measured in myocardial tissue homogenate supernatants using the kits. Briefly, MDA concentrations were measured at 450 nm using a microplate reader (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions. The ROS content was detected using a fluorescence enzyme marker (Thermo Fisher Scientific) at an excitation wavelength of 500 nm and an emission wavelength of 525 nm. The SOD content was measured at 550 nm using an Ultraviolet visible spectrophotometer (Thermo Fisher Scientific).
E bc k019 s
Manufactured by Elabscience
E-BC-K019-S is a laboratory equipment product from Elabscience. It is designed for performing general laboratory tasks and procedures. The core function of this product is to assist in the standardized and controlled operation of various laboratory experiments and analyses.
Automatically generated - may contain errors
2 protocols using e bc k019 s
1
Oxidative Stress Evaluation in Myocardium
2
MDA and SOD Content Analysis
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Cell supernatants were collected and detected for the contents of MDA (E-EL-0060c, Elabscience Biotechnology Co., Ltd) and SOD (E-BC-K019-S, Elabscience Biotechnology Co., Ltd) using biochemical kits.
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