Log In Sign up
The largest database of trusted experimental protocols

1 ml monoq column

Manufactured by GE Healthcare
Visit Supplier

The 1 mL monoQ column is a chromatography column designed for the purification and separation of proteins, peptides, and other biomolecules. This column utilizes anion exchange resin to capture and elute target analytes based on their charge properties. The column has a bed volume of 1 mL and is suitable for use with a variety of sample types and purification protocols.

Automatically generated - may contain errors

Lab products found in correlation

Kta pure system, by GE Healthcare (2 mentions) Amicon ultra 0.5 ml 10 kda filter, by Merck Group (2 mentions) Prism v8, by GraphPad (2 mentions)

2 protocols using 1 ml monoq column

1

In vitro cA4 cleavage assays of Card1 and Csm6

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro cA4 cleavage assays were performed by incubating 2 μM Card1 or Csm6 with 100 μM cA4 in 150 μL reaction buffer containing 20 mM Tris–HCl, pH 7.5, 50 mM KCl, 50 mM NaCl, at 37°C (for Card1) or 55°C (for Csm6) in a time course. At desired time points, the protein was separated by using an Amicon Ultra 0.5-ml 10-kDa filter (Millipore) at 10,000 × g for 20 min at 4°C. The filtered 100 μL reactions were analyzed using a 1 mL monoQ column (GE Healthcare) on an ÄKTA Pure system (GE Healthcare). Elution was performed with a linear gradient from 0.1 M to 1 M NaCl at a flow rate of 0.5 ml/min. The UV absorbance data recorded at 260 nm were employed, and final graphs were represented by GraphPad Prism v8.0.
Rostøl J.T., Xie W., Kuryavyi V., Maguin P., Kao K., Froom R., Patel D.J, & Marraffini L.A. (2021). The Card1 nuclease provides defence during Type III CRISPR immunity. Nature, 590(7847), 624-629.
+ Open protocol
+ Expand
2

In vitro cA4 cleavage assays of Card1 and Csm6

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro cA4 cleavage assays were performed by incubating 2 μM Card1 or Csm6 with 100 μM cA4 in 150 μL reaction buffer containing 20 mM Tris–HCl, pH 7.5, 50 mM KCl, 50 mM NaCl, at 37°C (for Card1) or 55°C (for Csm6) in a time course. At desired time points, the protein was separated by using an Amicon Ultra 0.5-ml 10-kDa filter (Millipore) at 10,000 × g for 20 min at 4°C. The filtered 100 μL reactions were analyzed using a 1 mL monoQ column (GE Healthcare) on an ÄKTA Pure system (GE Healthcare). Elution was performed with a linear gradient from 0.1 M to 1 M NaCl at a flow rate of 0.5 ml/min. The UV absorbance data recorded at 260 nm were employed, and final graphs were represented by GraphPad Prism v8.0.
Rostøl J.T., Xie W., Kuryavyi V., Maguin P., Kao K., Froom R., Patel D.J, & Marraffini L.A. (2021). The Card1 nuclease provides defence during Type III CRISPR immunity. Nature, 590(7847), 624-629.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!