μ-cell mass in sections was determined by thresholding and quantitating insulin-positive areas in nonadjacent sections at 100-μm intervals throughout the pancreas. All images were captured using FLoid Cell Imaging Station-Demo Video (Invitrogen) and a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek). Color deconvolution was based on the orthonormal transformation of the original RGB images. For each cohort, we analyzed a minimum of 15 individual whole-pancreatic immunofluorescence slides. The image analysis was done using the ImageJ software. Quantification of β cell mass was performed as described previously30 (link).
Floid cell imaging station demo video
Manufactured by Thermo Fisher Scientific
The FLoid Cell Imaging Station is a compact, easy-to-use fluorescence imaging system designed for visualizing and capturing images of cells. It features a high-resolution digital camera, LED illumination, and pre-configured imaging protocols for common fluorescent dyes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using floid cell imaging station demo video
1
Quantifying Pancreatic β-cell Mass
2
Quantifying Pancreatic β-cell Mass
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!