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Dry objective

Manufactured by Leica
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The 20x dry objective is a high-performance lens designed for use with Leica lab equipment. It provides a magnification of 20x and is intended for general microscopy applications. The objective features a numerical aperture and working distance suitable for a variety of sample types and imaging requirements. Further details on the specific technical specifications and intended use cases are not available at this time.

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Lab products found in correlation

Close spelling variants of this product

HC PL APO CS2 20x/0.75 DRY objective HC PL Fluotar 10x/0.30 Dry objective HC PL APO 10x/0.40 Dry objective APO 20×/0.70 DRY PH2 objective HC PL FLUOTAR L 20x/0.40 DRY objective HCX PL Fluotar L40x/0.60 CORR dry objective HC PL APO CS2 ×20/0.75 DRY objective HC PL APO CS2 20× DRY objective lens HC PL FLUOTAR 0.5 NA 20.0×dry objective HCX PL APO 10X/0.4 NA dry objective

2 protocols using dry objective

1

Visualizing Zebrafish Embryo Angiogenesis

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For in vivo imaging live embryos were mounted in 1% low melting point agarose in E3 embryo medium with 168 mg/l Tricaine (1X) for anaesthetization and 0.003% phenylthiourea to inhibit pigmentation. Imaging was carried out at SP5 or SP8 (Inverted) confocal microscopes using a 20x dry objective (Leica Microsystems). A heated microscope chamber at 28.5°C was used for recording time lapse videos. Stacks were taken every 15-25 min with a step size of 2.0 μm. Confocal stacks and time lapse videos were analysed using IMARIS Software (Bitplane) and ImageJ (NIH). Vessels in close proximity to the CrDI and NCA were cropped for better visualization.
Hasan S.S., Tsaryk R., Lange M., Wisniewski L., Moore J.C., Lawson N.D., Wojciechowska K., Schnittler H, & Siekmann A.F. (2017). Endothelial Notch signalling limits angiogenesis via control of artery formation. Nature cell biology, 19(8), 928-940.
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2

Visualizing Zebrafish Embryo Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo imaging live embryos were mounted in 1% low melting point agarose in E3 embryo medium with 168 mg/l Tricaine (1X) for anaesthetization and 0.003% phenylthiourea to inhibit pigmentation. Imaging was carried out at SP5 or SP8 (Inverted) confocal microscopes using a 20x dry objective (Leica Microsystems). A heated microscope chamber at 28.5°C was used for recording time lapse videos. Stacks were taken every 15-25 min with a step size of 2.0 μm. Confocal stacks and time lapse videos were analysed using IMARIS Software (Bitplane) and ImageJ (NIH). Vessels in close proximity to the CrDI and NCA were cropped for better visualization.
Hasan S.S., Tsaryk R., Lange M., Wisniewski L., Moore J.C., Lawson N.D., Wojciechowska K., Schnittler H, & Siekmann A.F. (2017). Endothelial Notch signalling limits angiogenesis via control of artery formation. Nature cell biology, 19(8), 928-940.
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