Fat mass and lean mass were measured with an EchoMRI-500 whole body composition analyzer (Echo Medical Systems, LLC, Houston, TX, USA) according to the manufacturer’s instruction 3 days before rats were euthanized. The analyzer measures fat mass, lean mass, and free water in scanned specimens using quantitative magnetic resonance theory, and this method has been validated in rats [13 (link)]. A clear plastic container of canola oil with a similar weight to the experimental rat was placed in the machine and tested as a fat standard before measuring body composition. Individual live rats were placed in a clear holder. The holder was then placed into a tubular space in the EchoMRI system and activated through a software program. Fat is expressed as equivalent weight in canola oil. Lean is the muscle tissue mass equivalent of all body parts containing water, excluding fat, bone minerals, hair, claws, etc. Free water comes mostly from the bladder.
Echomri 500 whole body composition analyzer
Manufactured by Echo Medical Systems
Sourced in United States
The EchoMRI-500 is a whole body composition analyzer developed by Echo Medical Systems. It is designed to measure the body composition of individuals, including fat mass, lean mass, and total body water, using magnetic resonance imaging (MRI) technology.
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2 protocols using echomri 500 whole body composition analyzer
1
Quantitative Body Composition Analysis
2
Longitudinal Body Composition Analysis
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To monitor body weight gain, animals were weighed once a week during 16 months. Body composition analysis to determine fat contents was performed on conscious young and old mice, using the EchoMRI-500™ whole body composition analyzer (Echo Medical Systems). Before blooding sampling, WT and Tg(Gasp-1) mice were fasted 16 h. Blood samples were collected in lithium heparin tubes under isofluorane anesthesia following the recommendations of ethical guidelines. At the collection day, blood samples were centrifuged at 4000 rpm for 10 min and plasmas were harvested then analyzed on a KONELAB 30 automat as previously described [32] .
Muscle and adipose tissue weights were measured following dissection of young and old mice. Individual muscles (tibialis anterior, gastrocnemius and soleus) from both sides of the animal were taken and the average weight was used for each muscle.
Muscle and adipose tissue weights were measured following dissection of young and old mice. Individual muscles (tibialis anterior, gastrocnemius and soleus) from both sides of the animal were taken and the average weight was used for each muscle.
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