Primary antibodies: rabbit‐anti‐MAD2 (1:200; ab70385, abcam; or 1:500 ab70383, abcam); sheep‐anti‐tryptophan hydroxylase/TPH (1:150; ab32821, abcam); rabbit‐anti‐BubR1 (1:100; A300‐386A, Bethyl Laboratories Inc.); rabbit‐anti‐P31comet (murine) (1:100; provided by Dr. Hongtao Yu; UT Southwestern, TX, USA); mouse‐anti‐SERT (H‐45) (1:20; raised and provided by Dr. Egon Ogris, Max Perutz Labs, Vienna, Austria (Montgomery et al, 2014 (link))); rabbit‐anti‐SEC24C (1:100; provided by Dr. Randy Schekman, UC Berkeley, CA, USA); rabbit‐anti‐GM130 (1:200; ab52649, abcam); rabbit‐anti‐β‐COP (1:100; PA1‐061, Invitrogen); rabbit‐anti‐TGN38 (1:100; OST00228G‐500UG, Osenses) rabbit‐anti‐Rab7 (1:100; 9367S, Cell Signaling); rabbit‐anti‐Rab5 (1:100; 3547S; Cell Signaling); rabbit‐anti‐Rab11A (1:100; 71‐5300; Invitrogen); rabbit‐anti‐Rab4 (1:100; PA3912, Invitrogen); and rabbit‐anti‐LAMP1 (1:100, L1418; Sigma‐Aldrich). Secondary antibodies: Alexa Fluor 488 donkey‐anti‐rabbit IgG (1:500; A21206, Invitrogen); Alexa Fluor 555 donkey‐anti‐sheep IgG (1:500; A21436, Invitrogen); Alexa Fluor 555 donkey‐anti‐mouse (1:500; A31570, Invitrogen); and Alexa Fluor 647 goat‐anti‐rabbit (1:500; A32733, Invitrogen).
Ab70383
Ab70383 is a monoclonal antibody that specifically recognizes the human Programmed Cell Death 1 (PD-1) protein. PD-1 is a cell surface receptor that plays a crucial role in regulating the immune system.
Lab products found in correlation
2 protocols using ab70383
Immunofluorescence Localization of Cell Cycle Proteins
Primary antibodies: rabbit‐anti‐MAD2 (1:200; ab70385, abcam; or 1:500 ab70383, abcam); sheep‐anti‐tryptophan hydroxylase/TPH (1:150; ab32821, abcam); rabbit‐anti‐BubR1 (1:100; A300‐386A, Bethyl Laboratories Inc.); rabbit‐anti‐P31comet (murine) (1:100; provided by Dr. Hongtao Yu; UT Southwestern, TX, USA); mouse‐anti‐SERT (H‐45) (1:20; raised and provided by Dr. Egon Ogris, Max Perutz Labs, Vienna, Austria (Montgomery et al, 2014 (link))); rabbit‐anti‐SEC24C (1:100; provided by Dr. Randy Schekman, UC Berkeley, CA, USA); rabbit‐anti‐GM130 (1:200; ab52649, abcam); rabbit‐anti‐β‐COP (1:100; PA1‐061, Invitrogen); rabbit‐anti‐TGN38 (1:100; OST00228G‐500UG, Osenses) rabbit‐anti‐Rab7 (1:100; 9367S, Cell Signaling); rabbit‐anti‐Rab5 (1:100; 3547S; Cell Signaling); rabbit‐anti‐Rab11A (1:100; 71‐5300; Invitrogen); rabbit‐anti‐Rab4 (1:100; PA3912, Invitrogen); and rabbit‐anti‐LAMP1 (1:100, L1418; Sigma‐Aldrich). Secondary antibodies: Alexa Fluor 488 donkey‐anti‐rabbit IgG (1:500; A21206, Invitrogen); Alexa Fluor 555 donkey‐anti‐sheep IgG (1:500; A21436, Invitrogen); Alexa Fluor 555 donkey‐anti‐mouse (1:500; A31570, Invitrogen); and Alexa Fluor 647 goat‐anti‐rabbit (1:500; A32733, Invitrogen).
Immunofluorescence Staining of Mitotic Markers
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