Polystyrene petri dishes

Manufactured by Corning

Sourced in United Kingdom

Polystyrene petri dishes are a type of laboratory equipment used for culturing cells, microorganisms, and other biological samples. They provide a flat, sterile surface for growth and observation. The dishes are made of a clear, disposable polystyrene material and come in various sizes to accommodate different experimental needs.

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Lab products found in correlation

Pluronic f 127, by Merck Group (2 mentions) Protein a g, by Thermo Fisher Scientific (2 mentions) Sdf 1α, by R&D Systems (2 mentions) Flexiperm gasket, by Merck Group (2 mentions) Icam 1 fc, by R&D Systems (2 mentions) E selectin fc chimera, by R&D Systems (1 mentions) P selectin fc chimera, by R&D Systems (1 mentions) Nih3t3 cells, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Petri dishes (74 citations) Petri dish (43 citations) Polystyrene Petri dish (2 citations)

4 protocols using polystyrene petri dishes

Engineered Surfaces for Cell Adhesion

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Non-tissue culture treated polystyrene petri dishes (Corning, Corning, NY) were enclosed using a single well FlexiPerm gasket (Sigma-Aldrich, St. Loius, MO). A solution of 2 μg mL⁻¹ of Protein A/G (Themo-Fisher Scientific, Waltham, MA) and 1 μg mL⁻¹ of SDF-1α (R&D Systems, Minneapolis, MN) was applied and incubated overnight at 4°C. The surfaces were then washed three times with PBS before a 30 minute incubation of a 0.2% w/v solution of Pluronic F-127 (Sigma-Aldrich). The surfaces were washed again three times with PBS. Next, a solution of ICAM-1/Fc and/or E-selectin/Fc chimera (R&D Systems) was applied to the surface and incubated for three hours at room temperature. The completed surfaces were then washed again three times with PBS before use. For experimentally tested points, molar ratios of 1:0, 10:1, 1:1, 1:10, and 0:1 E-selectin/Fc:ICAM-1/Fc were chosen to highlight differences between similar surfaces. In addition, total protein concentrations were chosen to yield total site densities of 4–5, 35–45, 130–160, 310–360, 550–610, and 1200–1300 sites/μm².

Anderson N.R., Lee D, & Hammer D.A. (2018). An Experimentally Determined State Diagram for Human CD4+ T Lymphocyte CXCR4-Stimulated Adhesion Under Shear Flow. Cellular and Molecular Bioengineering, 11(2), 91-98.

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Functionalized Surfaces for Cell Adhesion

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Non-tissue culture treated polystyrene petri dishes (Corning, Corning, NY) were enclosed using a single well FlexiPerm gasket (Sigma-Aldrich, St. Loius, MO). A solution of 2 µg mL⁻¹ of Protein A/G (Thermo-Fisher Scientific, Waltham, MA) and 1 µg mL⁻¹ of SDF-1α (R&D Systems, Minneapolis, MN) was applied and incubated overnight at 4°C. The surfaces were then washed three times with PBS before a 30-minute incubation of a 0.2% w/v solution of Pluronic F-127 (Sigma-Aldrich). The surfaces were washed again three times with PBS. Next, a solution of 8 µg/mL ICAM-1/Fc and 2 µg/mL P-selectin/Fc chimeras (R&D Systems) was applied to the surface and incubated for three hours at room temperature. The completed surfaces were then washed again three times with PBS before use.

Anderson N.R., Lee D, & Hammer D.A. (2019). Adhesive Dynamics simulations quantitatively predict effects of kindlin-3 deficiency on T-cell homing. Integrative biology : quantitative biosciences from nano to macro, 11(6), 293-300.

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Cell Culture Protocols for HeLa, NIH/3T3, and Jurkat

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HeLa CCL-2 cells and NIH/3T3 cells (ATCC, Manassas, VA) were grown under standard incubation conditions at 37 °C and 5% CO₂ in sterile-filtered DMEM with 10% heat-inactivated FBS, 2.5% HEPES, 1% Glutamine, and 1% Penicillin/Streptomycin (all Gibco). Jurkat cells were grown under the same conditions described above using RPMI 1640 complete medium instead of DMEM. Cells were plated onto T-75 flasks at 20% confluence and passaged every 3 days. For live and fixed-cell imaging experiments, cells were plated onto cell culture treated polystyrene Petri dishes (Corning).

Roxbury D., Jena P.V., Shamay Y., Horoszko C.P, & Heller D.A. (2015). Cell Membrane Proteins Modulate the Carbon Nanotube Optical Bandgap via Surface Charge Accumulation. ACS nano, 10(1), 499-506.

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Design and Validation of Nanovibrational Stimulation Apparatus

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The nanovibrational stimulation apparatus was nominally identical to that used by both Nikukar et al and Curtis et al (25, 26) . To perform experiments with 35 mm Petri dishes, six aluminium support discs were cut to 32 mm diameter, 3 mm thickness, polished (to ensure a smooth bonding surface). Six 35 mm tissue culture treated polystyrene Petri dishes (Corning, UK) were bonded onto the six aluminium discs using Loctite 2-part epoxy (Loctite, Hempstead, UK). Each cultureware assembly was subsequently bonded to a piezo transducer (model no. 010-05H Physik Instrumente, Karlsruhe/Palmbach, Germany) by application of a non-solvent glue (Bostik, UK). The transducers provided the required nanoscale amplitudes when driven by a continuous sine wave output from a GWINSTEK AFG-2005 arbitrary function generator (Good Will Instrument Euro B.V., Velhoven, Netherlands). The functionality of the piezo/cultureware assemblies was verified by incrementally driving each one at an audible frequency, i.e. 5 kHz, and listening to the audible output generated. The final set-up of the nanovibrational stimulation apparatus is shown in Figure 1.

Robertson S.N., Childs P.G., Akinbobola A., Henriquez F.L., Ramage G., Reid S., Mackay W.G, & Williams C. (2020). Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration. Journal of bioscience and bioengineering, 129(3).

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