We used the following primary antibodies: mouse-anti-RhoA (26C4, Santa Cruz, Heidelberg, Germany, sc-418), mouse-anti-Rac1 (BD Transd. Laboratories, Heidelberg, Germany, 610650), mouse-anti-p190RhoGAP (p190A) (BD Transd. Laboratories, 610149), mouse-anti-p190B (BD Transd. Laboratories, 611612), mouse-anti-RGS3 (CC-Q7, Santa Cruz, sc-100762), mouse-anti-caveolin-3 (BD Transd. Laboratories, 610421), mouse immunoglobulin G (IgG) (Santa Cruz sc-2025), rabbit-anti-caveolin-3 (H-100, Santa Cruz, sc-28828), mouse-anti-eNOS (BD Transd. Laboratories, 610297), rabbit-anti-phospho-1177Ser-eNOS (Cell Signaling Technology, Frankfurt, Germany, #9571), rabbit-anti-Nitrotyrosine (Millipore, Darmstadt, Germany, 06-284). The corresponding horseradish peroxidase-conjugated secondary antibodies were from Sigma-Aldrich (Munich, Germany, A-9044, A-9169). In this study, the following reagents and inhibitors were used: carbamoylcholine chloride (carbachol, Sigma-Aldrich, Munich, Germany, C4382), 5-bromo-2′-deoxyuridine (5-BrdU) (Sigma-Aldrich), methyl-β-cyclodextrin (MβCD, Sigma-Aldrich, M7439), (2S)-2-amino-5-(1-aminoethylideneamino)pentanoic acid; dihydrochloride (L-NIO dihydrochloride, Santa Cruz, sc-361229), Nω-nitro-L-arginin-methylester-hydrochloride (L-NAME, Sigma-Aldrich, 72760).
Mouse immunoglobulin g igg
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Mouse immunoglobulin G (IgG) is a type of antibody found in the blood and other bodily fluids of mice. It is a key component of the adaptive immune system, playing a crucial role in the recognition and neutralization of foreign pathogens.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti nitrotyrosine, by Merck Group (1 mentions)
Carbamoylcholine chloride carbachol, by Merck Group (1 mentions)
Mouse anti rhoa 26c4, by Santa Cruz Biotechnology (1 mentions)
Mouse anti rgs3 cc q7, by Santa Cruz Biotechnology (1 mentions)
Mouse anti rac1, by Santa Cruz Biotechnology (1 mentions)
Mouse anti enos, by Santa Cruz Biotechnology (1 mentions)
5 bromo 2 deoxyuridine 5 brdu, by Merck Group (1 mentions)
Mouse anti caveolin 3, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Merck Group (1 mentions)
Methyl β cyclodextrin mβcd, by Merck Group (1 mentions)
Human anti ago2 antibody, by Merck Group (1 mentions)
Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions)
2 protocols using mouse immunoglobulin g igg
1
Investigating Rho GTPase Signaling Pathways
2
MALAT1 Sponges miR-129-5p via RIP
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The RIP experiments were conducted using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the reference. Brie y, we lysed MALAT1 stable over-expressed HEC50 and HEC1A cells using RIPA buffer to extract the protein. Then human anti-Ago2 antibody (Millipore) or mouse immunoglobulin G (IgG, Santa Cruz Biotechnology, CA, USA) was incubated with the cellular extracts overnight at 4°C. After digested samples with proteinase K, we isolated the immunoprecipitated RNA and detected MALAT1 and miR-129-5p level using QRT-PCR method to further verify the sponge function of MALAT1 on miR-129-5p.
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