Transmission electron microscopy (TEM) was used to observe the microstructure of peptides with a Hitachi HT7700 (120 kV) Bio-TEM. The 0.05 % (w/v) RADA16-QLK before and after microbial transglutaminase (mTG) crosslinking were prepared at pH 7. The TEM samples were prepared by dropping 10 μL of peptide solution on the copper grid coated with carbon film and stained with 2 % phosphotungstic acid solution. The morphology of the peptide scaffolds was captured using atomic force microscopy (AFM) (DI Dimension 3100, Veeco Instruments Inc, US) in dynamic force mode. In brief, 0.05 % (w/v) peptide solution was sprayed on the surface of a cleaved mica for 5 min, the surface was then washed with sterilized water for several times and blown dry by anhydrous nitrogen.
Di dimension 3100
Manufactured by Veeco
Sourced in United States
The DI Dimension 3100 is a laboratory equipment designed for precise dimensional measurements. It features advanced optical imaging technology to capture and analyze the topography and surface characteristics of various samples. The core function of the DI Dimension 3100 is to provide accurate and reliable dimensional data for research, quality control, and process optimization applications.
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2 protocols using di dimension 3100
1
Microstructural Characterization of Peptide Scaffolds
2
Characterization of Black Phosphorus Nanomaterials
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Renishaw Raman Microscope 1000 (with 632.8 nm light) was employed to characterize Raman spectra of BP material. The thickness of BP nanosheets and BP deposited overlay were measured by AFM (Veeco Instruments Inc., di Dimension 3100). The surface coverage of BP-fiber was measured by SEM (Hitachi, S-520).
For optical interrogation system, broadband light source (BBS: Agilent HP83437A, Agilent Technologies Inc.) was used along with an optical spectrum analyzer (OSA, Agilent HP86142A, Agilent Technologies Inc.). The OSA was connected to a computer and the optical spectra were recorded by a customized program. Data analysis was performed using the customized program which automatically defined resonance wavelength using the centroid calculation method.
All biochemical procedures were performed in a fume cupboard. To minimize the cross-sensitivities of temperature and bend, all experiments were conducted at a controlled room temperature of 22.0 ± 0.1 °C unless specified otherwise, and the fiber device was placed straightly in a custom-made microchannel container and all the chemicals and solvents were added and withdrawn by careful pipetting.
For optical interrogation system, broadband light source (BBS: Agilent HP83437A, Agilent Technologies Inc.) was used along with an optical spectrum analyzer (OSA, Agilent HP86142A, Agilent Technologies Inc.). The OSA was connected to a computer and the optical spectra were recorded by a customized program. Data analysis was performed using the customized program which automatically defined resonance wavelength using the centroid calculation method.
All biochemical procedures were performed in a fume cupboard. To minimize the cross-sensitivities of temperature and bend, all experiments were conducted at a controlled room temperature of 22.0 ± 0.1 °C unless specified otherwise, and the fiber device was placed straightly in a custom-made microchannel container and all the chemicals and solvents were added and withdrawn by careful pipetting.
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