Male C57BL/6J wild type mice (14–16 weeks of age, Janvier, Le Genest Saint Ilsle, France) were subjected to I/R (n = 20) and sham operation (n = 16) as described below. Continuous electrocardiogram (ECG) monitoring was conducted by subcutaneously implanted telemetric devices one week before baseline recording in I/R (n = 10) and sham mice (n = 6). By serial transthoracic echocardiography, left ventricular (LV) function was assessed in all animals. Finally, an invasive electrophysiological study (EPS, details below) was performed 7 days after the index procedure, with a subset of n = 6 animals of each group receiving EPS already after 2 days. Hearts were excised for histologically determining the size of ischemic injury after EPS (for details see below). For peri- and postoperative analgesia mice received buprenorphine s.c. (0.1 mg/kg bodyweight). After final in vivo electrophysiological study, mice were exsanguinated under deep sedation with ketamine (100 mg/kg bodyweight) and xylazine (10 mg/kg bodyweight).
Male c57bl 6j wild type mice
Manufactured by Janvier Labs
Sourced in France
Male C57BL/6J wild type mice are an inbred mouse strain commonly used in biomedical research. These mice are genetically unmodified and serve as a standard control or reference population.
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4 protocols using male c57bl 6j wild type mice
1
Cardiac Ischemia-Reperfusion Injury in Mice
2
Aging and Alzheimer's Mouse Models
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Male C57BL/6J wild-type mice at 2, 8 and 22 months of age were obtained from Janvier (Janvier labs, Le Genest-Saint-Isle, France). Female 3 and 10 months old homozygous APP NL-G-F knock in mice and their wild-type C57BL/6J (WT) controls were available at our department at KI. Male 22 months old Cyp27A1 overexpressing mice and their littermate WT controls were available in our group. Mice were kept under controlled temperature (21°C) and humidity (55%) on a 12-h light-dark cycle and food/water were provided ad libitum.
3
Aging in Mouse and Lemur Models
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Two, 12 and 18 months-old C57BL/6J wild-type male mice were purchased from Janvier (Le Genest-Saint-Isle, France) and kept in ventilated cages under a 12:12 h light-dark cycle. Chow and water were available ad libitum. Male and female lemurs (M. murinus) 3–9 years old were born and raised in the laboratory colony of UMR 7179 (CNRS/MNHN, France, license approval A91.114.1). Animals were maintained in cages equipped with branches and wooden nests at constant temperature (25°C–27°C) and humidity (55%). Lemurs were exposed to the winter-like short-day-length photoperiod (10:14 h light-dark cycle). In the Brunoy colony, the animal median age was estimated to be 4.9 years for females and 5.7 years for males (Languille et al., 2012 (link)). Therefore, the senescence of animals was established from 6 years, associated with increased energy loss (body mass, resting metabolism) (Djelti et al., 2016 (link); Perret, 1997 (link); Pifferi et al., 2018 (link)). All experiments were approved by the Comité d’éthique Cuvier (n° 68) ethical board (authorization 22530-2019100917156614 and 13064-2018011615434702 ) and performed in strict accordance with European Directive 2010/63/EU.
4
PolG Mice Adaptation Protocol
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The studies described in this publication were approved by the Swiss Cantonal Veterinary Authority of Basel City, Switzerland, under the license numbers BS-2841 and BS-2794. C57BL/6J wild-type male mice were commercially purchased from Janvier Labs (Le Genest-Saint-Isle, France); B6.129S7(Cg)-Polgtm1Prol/J (PolG) male mice were obtained from The Jackson Laboratories and generated by the NIBR Cambridge LAS Transgenic Breeding Services group (Novartis Pharma, Cambridge, MA, USA). All the animals were allowed to adapt for 7 days prior to the start of the experiment and housed in IVC racks (max. 4 mice/XJ Type cage). The animals were given access to food and water ad libitum.
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