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2 protocols using ammonium peroxodisulfate aps

1

Synthesis of Photosensitive Acrylamide Polymers

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N,N-Dimethylacrylamide (DMAA) and CDCl3 were purchased from FUJIFILM Wako Pure Chemical Corporation. N,N-Diethylacrylamide (DEAA) and 4-acrylloylmorpholine (ACMO) were purchased from KJ Chemicals Corporation. Ammonium peroxodisulfate (APS) and 2,2’-azobis(isobutyronitrile) (AIBN) were purchased from Nacalai Tesque Inc. Deuterium oxide (D2O) was purchased from Sigma–Aldrich (Louis, MO, USA). The water used for the preparation of the aqueous solutions was purified with a Milli-Q ® Integral MT system. Other reagents were used without further purification. Azobenzene acrylamide (AzoAAm) was prepared according to a previous report [51 (link)].
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2

Synthesis and Characterization of βCD-modified Acrylamide Hydrogel

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Acrylamide (AAm), CDCl3, and D2O were purchased from Wako Pure Chemical Industries, Ltd. βCD was obtained from Junsei Chemical Co., Ltd. Triethylamine (Et3N), sodium hydroxide, hydrochloric acid, ammonium peroxodisulfate (APS), N,N,N′,N′–tetramethylethylenediamine (TEMED), and N,N′–methylenebis(acrylamide) (MBAAm) were purchased from Nacalai Tesque Inc. Acryloyl chloride and vinyltrimethoxysilane were purchased from Tokyo Chemical Industry Co., Ltd. DMSO–d6 was obtained from Merck & Co., Inc. RPMI-1640 medium, fetal bovine serum, penicillin and streptomycin were purchased from Sigma-Aldrich, Co. LifeAct-TagGFP2 and the Torpedo® lipofection reagent were purchased from ibidi GmbH. A highly porous synthetic resin (DIAION HP–20) used for column chromatography was purchased from Mitsubishi Chemical Co., Ltd. Water used for the preparation of the aqueous solutions was purified with a Millipore Integral MT system. Other reagents were used without further purification.
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