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Epr1535 2

Manufactured by Abcam

EPR1535(2) is a laboratory equipment product. Its core function is to detect and analyze electron paramagnetic resonance (EPR) signals.

Automatically generated - may contain errors

2 protocols using epr1535 2

1

Histological Analysis of Mouse Tissues

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Mouse tissues were dissected and fixed in 4% paraformaldehyde for histology and IHC analysis. For staining, the tissues were embedded in paraffin according to standard procedures. 5 μm sections were cut and processed for histology or immunostaining. The following primary antibodies were used: 4-hydroxynonenal (Genox, MHN-020P, 1:500), COX-2 (Cell Signaling Technology, D5H5, 1:400), AR [Abcam, EPR1535(2), 1:250], CK8 (Abcam, EP1628Y, 1:250), SYP (Abcam, YE269, 1:400), and Ki67 (Thermo Fisher Scientific, SP6, 1:100). The stained slides were visualized by a bright-field microscope.
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2

Immunohistochemical and Immunofluorescence Markers

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The following antibodies were used in these studies: anti-SOX2 (Abcam, ab236557), anti-AR (Abcam, EPR1535(2)), anti-Cytokeratin 5 (Abcam, ab52635; For IHC), anti-CK5 (Biolegend, 905904; For IF), anti-SYP (Cell Signaling Technology, #36406), and anti-K18 (ProteinTech, 66187-1-Ig).
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