Cells were harvested at 107 cells/ml by filtration onto 0.45 μm pore size nitrocellulose filters (Whatman), using vacuum, scraped from the membrane and immediately submerged in liquid nitrogen. The frozen cell, was cryogenically pulverized for six cycles of 15 Hz for 30 sec on a Retsch MM301 mixer mill. Sample chambers were pre-chilled in liquid nitrogen and re-chilled between each cycle.
0.45 μm pore size nitrocellulose filters
Manufactured by Cytiva
The 0.45 μm pore size nitrocellulose filters are a type of laboratory filtration equipment. They are designed to filter and separate particles, cells, or other materials from liquid samples based on their size, with a pore size of 0.45 micrometers.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 0.45 μm pore size nitrocellulose filters
1
Cryogenic Cell Pulverization Protocol
2
Protein-RNA Complex Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the filter binding assays, 2 CPS/µL (counts per second) radiolabeled probe was mixed with protein or protein mixture in a 100-µL reaction volume. After incubation for 45 min on ice, the mixture was filtered through Whatman 0.45-μm pore size nitrocellulose filters. Only protein-RNA complexes were retained on the filters and detected by scintillation counting. Data fits were performed with GraphPad Prism 5 using a single site saturation binding mode.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!