Goat anti mouse igg h l alexa fluor 594 antibody

Manufactured by Abcam

Goat anti-mouse IgG(H+L) (Alexa Fluor 594) antibody is a secondary antibody conjugated with the Alexa Fluor 594 fluorescent dye. It is designed to detect and bind to mouse immunoglobulin G (IgG) heavy and light chains.

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Lab products found in correlation

Fluorescence inversion microscope, by Nikon (2 mentions) Mouse anti e2 csfv antibody, by Abmart (2 mentions) Goat anti rabbit igg h l alexa fluor 488 antibody, by Abcam (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions)

Close spelling variants of this product

Goat anti-mouse IgG H&L (Alexa Fluor 594) Anti-mouse IgG H&L (Alexa Fluor® 594) Alexa Fluor 594 goat anti-mouse IgG Alexa Fluor 594 goat anti-mouse antibody Goat anti-mouse Alexa Fluor 594 Alexa Fluor 594 anti-mouse IgG Goat anti-mouse IgG H&L Anti-mouse Alexa Fluor 594 Goat anti-mouse Alexa 594 Alexa Fluor 594

3 protocols using goat anti mouse igg h l alexa fluor 594 antibody

CSFV Replication Quantification via Indirect IFA

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CSFV replication (Fig. 4B, Fig. 5C, and Fig. 7B) was measured using an indirect IFA according to a method described previously (17 (link)). Briefly, CSFV-infected cells or inhibitor-treated cells were fixed with 4% paraformaldehyde for 20 min at room temperature and washed three times with cold PBS. After fixation, the cells were permeabilized with 0.2% Triton X-100 for 10 min at room temperature and washed three times with PBS. After blocking with 3% BSA for 2 h, the cells were incubated with mouse anti-E2 CSFV antibody (1:200) (Ab-mart) at room temperature for 2 h. After three washes with PBS, the cells were incubated with goat anti-mouse IgG(H+L) (Alexa Fluor 594) antibody (1:200) (catalog number ab150116; Abcam) for 1 h at 37°C. Fluorescence-positive wells were observed using a fluorescence inversion microscope (Nikon, Tokyo, Japan).

Zhang L., Wang T., Yi Y., Song M., Jin M., Guo K, & Zhang Y. (2022). ARF1 with Sec7 Domain-Dependent GBF1 Activates Coatomer Protein I To Support Classical Swine Fever Virus Entry. Journal of Virology, 96(6), e02193-21.

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Indirect IFA for CSFV Titration

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An indirect IFA was used for viral titration using a previously described method (17 (link)). Briefly, cells were seeded into 96-well plates and inoculated with CSFV-containing cell supernatants for 72 h in an incubator at 37°C with 5% CO₂. Next, the cells were fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature and washed with cold phosphate-buffered saline (PBS) three times. After fixation, the cells were permeabilized with 0.2% Triton X-100 for 10 min at room temperature and washed three times with PBS. After blocking with 3% bovine serum albumin (BSA) for 2 h, the cells were incubated with mouse anti-E2 CSFV antibody (1:200) (Ab-mart) at room temperature for 2 h. After three washes with PBS, the cells were incubated with goat anti-mouse IgG(H+L) (Alexa Fluor 594) antibody (1:200) (catalog number ab150116; Abcam) for 1 h at 37°C. Fluorescence-positive wells were observed and recorded using a fluorescence inversion microscope (Nikon, Tokyo, Japan). Viral titration was determined as the 50% tissue culture infectious dose (TCID₅₀) per milliliter using the method proposed by Reed and Muench (48 ).

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Primary Human Mesenchymal Stem Cell Culture

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Alpha minimum essential medium (MEM alpha (1×)+Gluta MAXTM-1), l-glutamine, and penicillin/streptomycin solution were obtained from Thermo Fisher Scientific (Waltham, MA). Fetal bovine serum (FBS) was purchased from Gemini Bioproducts (West Sacramento, CA). DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride) was purchased from Anaspec Inc. (Fremont, CA). Phosphate buffered saline (PBS), without calcium and magnesium, and trypsin/ ethylenediaminetetraacetic acid (EDTA) (1× 0.25% trypsin/2.21 × 10⁻³_M EDTA in Hank’s balanced salt solution without sodium bicarbonate, calcium, and magnesium) were supplied by Mediatech Inc. (Manassas, VA). Primary hMSCs were obtained from healthy consenting donors at the Texas A&M Health Science Center, Institute for Regenerative Medicine. Mouse anti-tubulin β 3 (TUBB3) antibody was purchased from BioLegend (San Diego, CA). Rabbit anti-neurofilament heavy polypeptide (NHP) antibody, goat anti-mouse IgG-H&L (Alexa Fluor 594) antibody, and goat anti-rabbit IgG-H&L (Alexa Fluor 488) antibody were purchased from Abcam (Cambridge, UK). Unless otherwise listed, all solvents and reagents were purchased from Aldrich Chemical Co. (St. Louis, MO) and used as received.

Miao S., Cui H., Nowicki M., Xia L., Zhou X., Lee S.J., Zhu W., Sarkar K., Zhang Z, & Zhang L.G. (2018). Stereolithographic 4D Bioprinting of Multiresponsive Architectures for Neural Engineering. Advanced biosystems, 2(9), 1800101.

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