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Meoh lc ms grade

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MeOH (LC-MS grade) is a high-purity solvent used in liquid chromatography-mass spectrometry (LC-MS) applications. It is designed to meet the stringent requirements of LC-MS analysis.

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Lab products found in correlation

Acetylcholine chloride, by Merck Group (1 mentions) Hclo4, by Merck Group (1 mentions) γ aminobutyric acid, by Merck Group (1 mentions) Hplc grade, by Thermo Fisher Scientific (1 mentions) Formic acid, by Fujifilm (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) L lactic acid, by Merck Group (1 mentions) Uplc q tof ms, by Waters Corporation (1 mentions) Uplc tqms, by Waters Corporation (1 mentions) Milli q water system, by Merck Group (1 mentions) Acetyl chloride, by Merck Group (1 mentions) Disodium hydrogen phosphate, by Carl Roth (1 mentions) Acetic acid, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) N hexane, by Carl Roth (1 mentions) Potassium carbonate, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions)

4 protocols using meoh lc ms grade

1

Quantification of Neurotransmitters and Analogs

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Dopamine hydrochloride (Alfa Aesar, Kandel, Germany) and 2-(3,4-dihydroxy-phenyl)ethyl-1,1,2,2-d4-amine HCl (DAd4) (CDN Isotopes, Pointe-Claire, Canada) were dissolved in 200 mM HClO4 (Sigma-Aldrich, Steinheim, Germany), whereas γ-aminobutyric acid (Sigma-Aldrich, Steinheim, Germany) and 4-aminobutyric-2,2,3,3,4,4-d6 acid (GABAd6) (EQ Laboratories GmbH, Augsburg, Germany) stock solutions were prepared in 10% methanol (MeOH) (LC-MS grade, Thermo Fisher Scientific, Waltham, MA, USA). Serotonin hydrochloride (Alfa Aesar), serotonin-α,α,β,β-d4 creatinine sulfate complex (SRTd4) (CDN Isotopes, Pointe-Claire, Canada), acetylcholine chloride (Sigma-Aldrich, Steinheim, Germany) and acetylcholine-1,1,2,2-d4 chloride (AChd4) (EQ Laboratories GmbH, Augsburg, Germany) were dissolved in bidistilled water. The deuterated analogue of the respective neurotransmitter was taken as an internal standard.
Weishaupt A.K., Kubens L., Ruecker L., Schwerdtle T., Aschner M, & Bornhorst J. (2023). A Reliable Method Based on Liquid Chromatography–Tandem Mass Spectrometry for the Simultaneous Quantification of Neurotransmitters in Caenorhabditis elegans. Molecules, 28(14), 5373.
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2

SGJD Compound Analysis by LC-MS/MS

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Eleven reference standard compounds (Figure S1) for the LC-MS/MS analysis of the SGJD were purchased from commercial suppliers: compounds Co., Ltd. (Wuhan, China). Methanol (MeOH; LC-MS-grade) and acetonitrile (ACN; HPLCgrade) were purchased from ThermoFisher Scientific (San Jose, CA, USA) and J.T. Baker (Phillipsburg, NJ, USA), respectively. Distilled water (DW) was produced with a Vivagen water purification system (EXL3 Analysis 16, Seongnam, Korea). Formic acid (FA; ≥99.5%) and ammonium formate (AF; 99.0%) were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan) and Kanto Chemical Co., Inc. (Tokyo, Japan), respectively.
Seo C., & Shin H. (2021). Liquid Chromatography Tandem Mass Spectrometry for the Simultaneous Quantification of Eleven Phytochemical Constituents in Traditional Korean Medicine, Sogunjung Decoction. Processes, 9(1), 153-153.
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3

Quantitative Lipidomic Analysis via UPLC-MS

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LC–MS grade MeOH, MeCN, and formic acid were purchased from Fisher Scientific (Loughborough, UK). Ultra-pure water was purified by a Milli-Q water system (Millipore, Milford, MA, USA). Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, USA), physiological saline (0.9% sodium chloride solution).
l-Lactic acid (CAS: 79-33-4, purity ≥ 98%), l-Valine (CAS: 72-18-4, purity ≥ 9%), l-Phenylalanine (CAS: 63-91-2, purity ≥ 98%), Arachidonic acid (CAS: 506-32-1, purity ≥ 98%), Stearic acid (CAS: 57-11-4, purity ≥ 98%), Linoleic acid(CAS: 60-33-3, purity ≥ 98%), Palmitic acid (CAS: 57-10-3, purity ≥ 98%), Oleic acid (CAS: 112-80-1, purity ≥ 98%), LysoPC (18:0) (CAS: 19420-57-6, purity ≥ 98%), LysoPC(16:0) (CAS: 17364-16-8, purity ≥ 98%), LysoPC (18:1(9Z)) (CAS: 19420-56-5, purity ≥ 98%), Heptadecanoic acid (IS, CAS: 506-12-7, purity ≥ 98%) and 19:0 LysoPC (IS, CAS: 108273-88-7, purity ≥ 98%) were purchased from Shanghai Yuan-ye Biological Technology Co., Ltd. (Shanghai, China).
Instruments used in this study include a vortex mixer (Haimen Kylin-Bell Lab Instruments Co., Ltd., Jiangsu, China), cryogenic super-centrifuge (Thermo Fisher Scientific, USA), nitrogen evaporator (Beijing Chengmeng Weiye Technology Co., Ltd., Beijing, China), UPLC-Q-TOF/MS (Waters Corp., Milford, MA, USA), UPLC-TQ-MS (Waters Corp., Milford, MA, USA).
Qin N., Qin M., Shi W., Kong L., Wang L., Xu G., Guo Y., Zhang J, & Ma Q. (2022). Investigation of pathogenesis of hyperuricemia based on untargeted and targeted metabolomics. Scientific Reports, 12, 13980.
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4

Lipidomic Analysis of Oxylipins

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HPLC-grade methanol (MeOH) and methyl tert-butyl ether (MTBE, Acros Organics) as well as LC-MS grade MeOH, acetonitrile (ACN) and acetic acid (HOAc) were purchased from Fisher Scientific (Schwerte, Germany). Ammonium acetate was purchased from Merck (Darmstadt, Germany), potassium carbonate, ethyl acetate and acetyl chloride were obtained from Sigma Aldrich (Schnelldorf, Germany). Disodium hydrogen phosphate and n-hexane were obtained from Carl Roth (Karlsruhe, Germany). Oxylipin standard substances and deuterated internal oxylipin standards were purchased from Cayman Chemicals via local distributor Biomol (Hamburg, Germany), E-series resolvins RvE2, 18(S)-RvE3 and 18(R)-RvE3 were a kind gift of the lab of Makoto Arita (RIKEN Center for Integrative Medical Sciences, Japan), methyl pentacosanoate (FAME C25:0) was obtained from Santa Cruz Biotechnology (Heidelberg, Germany). PCR primers were purchased from Cayman Chemicals via local distributor Biomol (Hamburg, Germany).
Kutzner L., Esselun C., Franke N., Schoenfeld K., Eckert G.P, & Schebb N.H. (2020). Effect of dietary EPA and DHA on murine blood and liver fatty acid profile and liver oxylipin pattern depending on high and low dietary n6-PUFA. Food & function, 11(10).
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