Cell light edu apollo 488 in vivo imaging kit

To detect the DNA synthesis of hepatocytes, NCTC 1469 or HL7702 cells were seeded in 96-well plates at a density of 1×10⁵/ml. 50 μM EdU was added into the medium and incubated for 2 hrs before the end of 48 hrs of cell treatment. After cells were washed 3 times with PBS and fixed with 4% paraformaldehyde (PFA) for 30 min, EdU staining was conducted using Cell-Light™ EdU Apollo®488 In Vivo Imaging Kit (RiboBio, China). Nuclei were counterstained with Hoechst (Sigma, USA). Digital images were acquired under fluorescence microscopy (Leica, Germany) with original magnification of 100x. The results were presented as EdU positive cell rate which was analyzed with ImageJ software.

Mice were injected intraperitoneally with 50 mg/kg of EdU (C10314-3; Riobio, Guangzhou, China) 1 hr before killed. After washed with PBS for 15 min., frozen sections were fixed in paraformaldehyde for 30 min. After fixed, frozen sections were washed with PBS for three times, and then incubated with Cell-Light™ EdU Apollo®488 In Vivo Imaging Kit (C10314-3; Riobio). After incubation with EdU kit, sections were washed with PBS for three times, then incubated with DAPI (4′,6-diamidino-2-phenylindole, 1:500 dilution; Life Technology, Carlsbad, CA, USA). The images were taken under an amplification of 400× with confocal laser scanning microscope (LSM 710; Carl Zeiss MicroImaging GmbH, Jena, Germany). The results were expressed as EdU-positive cell number per mm².