Pcb 138

The following equipment and materials have been used in this study: carbon dioxide (Air Liquide, Liège, Belgium); helium (Air Products, Brussels, Belgium); acetonitrile (Biosolve, Valkenswaard, Netherlands); luminometer Orion II (BRS, Drogenbos, Belgium); adenosine triphosphate (ATP), Dulbecco's modified Eagle's medium (DMEM), DMEM without red phenol, and fetal bovine serum and trypsin (Fisher Bioblock Scientific, Tournai, Belgium); Focus gas chromatograph and Polaris Q mass spectrometer (Interscience, Louvain-la-Neuve, Belgium); charcoal, dextran, o,p′-DDD, p,p′-DDD, p,p′-DDE, o,p′-DDT, p,p′-DDT, 17β-estradiol, HCB, α-HCH, β-HCH, γ-HCH, δ-HCH, nonane, PCB 80 ¹³C, PCB 138 (2,2′,3,4,4′,5′-hexachlorobiphenyl), PCB 153 (2,2′,4,4′,5,5′-hexachlorobiphenyl), and PCB 180 (2,2′,3,4,4′,5,5′-heptachlorobiphenyl) (Sigma-Aldrich, Bornem, Belgium); D-luciferin (potassium salt) (Synchem OHG, Kassel, Germany); HT8 column (VWR, Leuven, Belgium).

The PCB analytical standards with a purity degree more than 99% (PCB 180, 2,2′,3,4,4′,5,5′‐heptachlorobiphenyl; PCB 138, 2,2′,3,4,4′,5′‐hexachlorobiphenyl; PCB 153, 2,2′,4,4′,5,5′‐hexachlorobiphenyl; PCB 101, 2,2′,4,5,5′‐Pentachlorobiphenyl; PCB 52, 2,2′,5,5′‐tetrachlorobiphenyl and PCB 28, 2,4,4′‐trichlorobiphenyl) were obtained from Sigma‐Aldrich (USA). Methylbenzene, acetonitrile, methyl alcohol, MgSO₄, toluene, primary secondary amine (PSA), and NaCl were obtained from Merck Co. (Germany).