Paraformaldehyde-fixed, paraffin-embedded tissue sections were deparaffinized, subjected to heat-mediated antigen retrieval, blocked with normal serum, and stained with antibody against Pi4ka. Biotinylated anti-rabbit secondary antibody was followed by Avidin-Biotin Complex Elite and DAB Peroxidase Kit (Vector Laboratories). See antibodies in Supplemental Experimental Procedures . An Olympus DP73 camera and cellSens software were used to image non-fluorescent stains.
Avidin biotin complex elite
Manufactured by Vector Laboratories
Avidin-Biotin Complex Elite is a high-affinity reagent used for the detection and localization of biotinylated molecules. It consists of avidin conjugated to an enzyme or fluorescent label, providing a sensitive and versatile tool for various applications.
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Lab products found in correlation
2 protocols using avidin biotin complex elite
1
Immunohistochemical Detection of Pi4ka
2
Immunohistochemical Detection of Pi4ka
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