Victor x4 multifunctional microplate reader

Manufactured by PerkinElmer

Sourced in United States

The VICTORTM X4 Multifunctional Microplate Reader is a versatile lab equipment designed for various microplate-based assays. It offers high-performance detection capabilities, including absorbance, fluorescence, and luminescence measurements, enabling researchers to conduct a wide range of experiments.

Automatically generated - may contain errors

Lab products found in correlation

Stat matic plate washer 2, by Merck Group (4 mentions) Antioxidant assay kit, by Cayman Chemical (1 mentions)

Close spelling variants of this product

Microplate reader (409 citations) Victor X4 (200 citations) Victor microplate reader (38 citations) Multifunctional microplate reader (21 citations)

4 protocols using victor x4 multifunctional microplate reader

Quantifying Endocannabinoids in Rat Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

AEA and 2-AG concentrations were measured in rat brain homogenates with commercial ELISA kits (MyBioSource, San Diego, CA, USA). The sensitivity of the tests was 0.1 ng/mL and 0.43 pg/mL for AEA and 2-AG, respectively. The protocols were performed according to the manufacturers’ instructions. A Stat-Matic Plate Washer II (Sigma-Aldrich) was used for the washing steps. The optical density was measured at 450 nm, the protein concentration of the samples was determined by interpolation from the standard curve, and intra-assay %CV was 1.42 for AEA and 5.73 for 2-AG.
In BCA assay and ELISA, absorbance was read by VICTORTM X4 Multifunctional Microplate Reader (Perkin Elmer, Waltham, MA, USA), and the results were analyzed with WorkOut 2.5 Software. The mean concentration of protein per mL was determined with reference to a four-parameter logistic curve (4-PL).

Zubrzycki M., Zubrzycka M., Wysiadecki G., Szemraj J., Jerczynska H, & Stasiolek M. (2022). Effect of Fatty Acid Amide Hydrolase Inhibitor URB597 on Orofacial Pain Perception in Rats. International Journal of Molecular Sciences, 23(9), 4665.

+ Open protocol

+ Expand

Quantifying Endocannabinoids in Rat CSF

Check if the same lab product or an alternative is used in the 5 most similar protocols

The AEA and 2-AG concentrations were measured in rat CSF with commercial ELISA kits (MyBioSource, San Diego, CA, USA). The sensitivity of the tests was 0.1 ng/mL and 0.43 pg/mL for AEA and 2-AG, respectively. The protocols were performed according to the manufacturers’ instructions. A Stat-Matic Plate Washer II (Sigma-Aldrich) was used for the washing steps. The optical density was measured at 450 nm and the protein concentration of the samples was determined by interpolation from the standard curve; the intra-assay %CV was 1.42 for AEA and 5.73 for 2-AG.
Absorbance was read using a VICTOR^TM X4 Multifunctional Microplate Reader (Perkin Elmer, Waltham, MA, USA), and the results were analyzed with WorkOut 2.5 Software. The mean concentration of protein per mL was determined with reference to a four-parameter logistic curve (4-PL).

Zubrzycki M., Zubrzycka M., Wysiadecki G., Szemraj J., Jerczynska H, & Stasiolek M. (2022). Release of Endocannabinoids into the Cerebrospinal Fluid during the Induction of the Trigemino-Hypoglossal Reflex in Rats. Current Issues in Molecular Biology, 44(5), 2401-2416.

+ Open protocol

+ Expand

Biomarker Analysis of Surgical Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

The venous blood samples were collected the day prior to the surgery and on the first postoperative day, between the hours 07:00 and 09:00 a.m. The samples were centrifuged at 7000 rpm for 10 min and were refrigerated at −80 °C until the biomarkers analysis. For proteins measurement, commercially available ELISA kits and an Antioxidant Assay Kit (Cayman Chemical, Ann Arbor, MI) were used. Protocols were performed following the manufacturer’s instructions. The concentration of proteins in the samples was determined by interpolation from the standard curve. The absorbance was read by Multifunctional Microplate Reader VICTORTM X4 (Perkin Elmer, Waltham, MA). All ELISA results were analysed with WorkOut 2.5 Software and the mean concentration of protein per mL was determined by referring to the four-parameter logistic (4-PL) curve. For washing steps, the Stat-Matic Plate Washer II (Sigma-Aldrich, St. Louis, MO) was used. The investigators who performed the laboratory analysis were blinded to the patients’ clinical data.

Kaźmierski J., Miler P., Pawlak A., Jerczyńska H., Nowakowska K., Walkiewicz G., Woźniak K., Krejca M, & Wilczyński M. (2022). Increased postoperative myeloperoxidase concentration associated with low baseline antioxidant capacity as the risk factor of delirium after cardiac surgery. Annals of Medicine, 54(1), 610-616.

+ Open protocol

+ Expand

Serum Cortisol ELISA Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum cortisol level was determined with an ELISA kit based on competitive binding principle according to the manufacturer’s instructions (cat. no. DEH3388, Demeditec, Kiel, Germany). Standards, samples and horseradish peroxidase-labelled cortisol were added to microtiter wells coated with an anti-cortisol antibody. An unknown amount of cortisol from the sample competed with a cortisol–HRP conjugate for binding to the coated antibody. After the incubation step, any unbound substances were washed off. The amount of bound peroxidase conjugate was inversely proportional to the concentration of cortisol in the sample, therefore, after the addition of the substrate solution, the colour intensity was also inversely proportional to the cortisol concentration in the sample.
A Stat-Matic Plate Washer II (Sigma-Aldrich, Taufkirchen, Germany) was used for the washing steps in the ELISA assays and the absorbance was read via Multifunctional Microplate Reader VICTORTM X4 (Perkin Elmer, Shelton, CT, USA). All ELISA results were analysed with WorkOut 2.5 Software and the mean concentration of protein per ml was determined by interpolation from the standard curve referring to the four-parameter logistic (4-PL) curve.

Sobczak J., Burzyńska M., Sikora A., Wysocka A., Karawani J, & Sikora J.P. (2023). Post-Traumatic Stress Response and Appendicitis in Children—Clinical Usefulness of Selected Biomarkers. Biomedicines, 11(7), 1880.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!