The ABTS radical [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] scavenging capacity of the samples was determined as described previously [15] . The stock solution of ABTS radical consisted of 7 mM ABTS in potassium persulfate 2.45 mM, kept in the dark at room temperature for 16 h. An aliquot of stock solution was diluted in Milli-Q water to obtain a working solution of ABTS radical with an absorbance of 0.70 ± 0.02 at 734 nm. Twenty microliters of sample (25 mg/mL) were then mixed with 980 µL of ABTS reagent and then left to stand in the dark at 30 °C for 10 min. Absorbance values were read at 734 nm using a UV-1601 spectrophotometer (Model CPS-240, Shimadzu, Japan). Results were expressed as mg equivalents ascorbic acid /g of sample based on a standard curve of ascorbic acid.
Model cps 240
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model CPS-240 is a laboratory centrifuge designed for general-purpose applications. It features a maximum rotational speed of 6,000 rpm and a maximum relative centrifugal force of 3,300 x g. The centrifuge accommodates a variety of rotor configurations to handle various sample volumes and tube sizes.
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Lab products found in correlation
2 protocols using model cps 240
1
ABTS Radical Scavenging Assay
2
Spectrophotometric Quantification of Astaxanthin
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The lipid extracts were diluted with hexane and the absorbance was measured at 470 nm using a UV-1601 spectrophotometer (Model CPS-240, Shimadzu, Kyoto, Japan). The carotenoid concentration was calculated using the equation described by Britton (1995) , where A is absorbance, V is the dilution volume (mL), P is the molecular weight of astaxanthin (597), and ε is the molar absorption coefficient reported by Britton (1995) for astaxanthin (125100). Results are expressed as mg astaxanthin/100 mg lipid extract.
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