All procedures related to animal experimentation were reviewed and approved by the Kantonales Veterinäramt Basel‐Stadt. Swiss mice (Janvier) were group housed (weaning at postnatal day 21–23) under a 12‐h light/dark cycle (06:00–18:00) with food and water ad libitum. Dissociated cultures of neocortical cells were prepared from E16.5 mouse embryos (embryonic stage 16.5). Neocortices were dissociated by the addition of papain (130 units, Worthington Biochemical, LK003176) for 30 min at 37°C. Cells (45,000 cells/cm2) were maintained in neurobasal medium (Gibco, 21103‐049) containing 2% B27 supplement (Gibco, 17504‐044), 1% Glutamax (Gibco, 35050‐61), and 1% penicillin/streptomycin (Bioconcept, 4‐01F00‐H) for 14 days. The following reagents for pharmacological treatments were used (at the indicated concentrations and from the stated sources): DRB (50 μM, Sigma, D1916), triptolide (1 μM, Sigma, T3652), bicuculline (20 μM, Tocris, 0130), BDNF (50 ng/ml, Sigma, B7395), DHPG (10 μM, Tocris, 0805), DL‐AP5 (50 μM, Tocris, 3693), KN‐62 (10 μM, Tocris, 1277), U0126 (10 μM, Tocris, 1144), SRPIN340 (10 μM, Tocris, 5063) and KH‐CB19 (10 μM, Tocris, 4262).
Srpin340
Manufactured by Bio-Techne
SRPIN340 is a small molecule inhibitor that selectively targets the Ser/Arg-rich (SR) protein kinase SRPK1. It is commonly used in research applications to study the role of SRPK1 in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Kh cb19, by Bio-Techne (3 mentions)
Brain derived neurotrophic factor (bdnf), by Merck Group (1 mentions)
Dl ap5, by Bio-Techne (1 mentions)
Kn 62, by Bio-Techne (1 mentions)
Bicuculline, by Bio-Techne (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by BioConcept (1 mentions)
Lk003176, by Worthington (1 mentions)
U0126, by Bio-Techne (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Swiss mice, by Janvier Labs (1 mentions)
Volasertib, by Selleck Chemicals (1 mentions)
Barasertib, by Selleck Chemicals (1 mentions)
Vx 680, by Selleck Chemicals (1 mentions)
Zm447439, by Bio-Techne (1 mentions)
Gw843682x, by Bio-Techne (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gsk3326595, by MedChemExpress (1 mentions)
Thz531, by MedChemExpress (1 mentions)
3 protocols using srpin340
1
Dissociated Neocortical Cell Cultures
2
Inhibitors of Cell Signaling Pathways
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GSK-626616 (PubChem CID: 15981157) was obtained from Tocris Bioscience. DMSO was from Sigma-Aldrich (D2438). The following inhibitors were used in this study: GW 843682X (Tocris Bioscience, 2977), ZM 447439 (Tocris Bioscience, 2458), Barasertib (Selleck chemicals, S1147), Volasertib (Selleck chemicals, S2235), VX-680 (Selleck chemicals, S1048), TCA 2317 HCl (Tocris Bioscience, 4066), KHCB19 (Tocris Bioscience, 4262), KHCB19 (Calbiochem, Merck Millipore, 219511), TG003 (Tocris Bioscience, 4336), SRPIN340 (Santa Cruz, sc-394310) and SRPIN340 (Tocris Bioscience, 5063). Prior to inhibitor treatments, cells were washed and serum deprived for 2 hrs.
3
Cell Line Characterization and Treatment
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LNCaP, 22RV1, C4-2, and RWPE-1 cell lines were obtained from the American Tissue Culture Collection (ATCC) and PNT1 cell line was acquired from Sigma. LN95 cell line was kindly provided by Professor Stephen Plymate (University of Washington). LNCaP, 22RV1, C4-2 and PNT1 cells were maintained in RPMI medium supplemented with 10% fetal bovine serum (FBS). RWPE-1 cells were cultured in keratinocyte serum-free media, while LN95 cells were maintained in phenol red-free RPMI supplemented with 10% charcoal-stripped FBS. All cell lines were allowed to adhere for at least one day prior to the treatment with inhibitors. THZ531, OSMI-4, TG003 and GSK3326595 were obtained from MedChemExpress. KH-CB19, Pladienolide B and SRPIN340 were purchased from Tocris.
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