Agilent bioanalyzer 2100 dna 1000 kit

Libraries for two individuals from each Populus balsamifera L., P. tremula L., P. mexicana Wesmael., Salix nigra Marshall, S. exigua Nutt., and S. phlebophylla Andersson (Table S3) were prepared using the NEBNext Ultra II DNA Prep Kit following the published protocol for this kit (New England Biolabs, Ipswitch, MA, USA), and quantified using an Agilent Bioanalyzer 2100 DNA 1000 kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were pooled at equimolar concentrations into two pools of six prior to probe hybridization following the Arbor Biosciences myBaits protocol v 3.0.1 and Hale et al. (2020) . The hybridized samples were subsequently pooled at equimolar ratios and sequenced at the Texas Tech Center for Biotechnology and Genomics using a MiSeq with the Micro chemistry and 150 bp paired-end reads (Illumina, Inc., San Diego, CA, USA).

The sex of 24 males and 24 females of S. nigra was identified from flowering catkins in a wild population near Dickens Springs, TX, USA in April 2017. Leaf tissue was collected and dried on silica beads. DNA was extracted from leaf tissue using the Qiagen DNeasy Plant Minikit (Qiagen, Hilden, Germany) and fragmented using sonication with the Covaris E220 Focused Ultrasonicator (Covaris, Inc., Woburn, MA, USA). Libraries were prepared using the NEBNext Ultra II DNA Prep Kit (New England Biolabs, Ipswitch, MA, USA), and quantified using an Agilent Bioanalyzer 2100 DNA 1000 kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were pooled at equimolar concentrations into sixteen pools of six prior to probe hybridization to targeted capture probes following the Arbor Biosciences myProbes protocol v 3.0.1. The hybridized samples were subsequently pooled at equimolar ratios and sequenced at the Oklahoma Medical Research Foundation (Oklahoma, OK, USA) using a HiSeq 3000 (Illumina, Inc., San Diego, CA, USA).