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Ab76315

Manufactured by Thermo Fisher Scientific
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The Ab76315 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to perform sample preparation and analysis tasks. Further details about its specific capabilities and intended applications are not available.

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Lab products found in correlation

Tissue microarray tma, by Shanghai Outdo Biotech Co. (1 mentions) Matrigel, by Corning (1 mentions) Transwell permeable supports with 8μm pore, by Corning (1 mentions)

2 protocols using ab76315

1

Breast Cancer Tissue Microarray Analysis

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Tissue microarray (TMA) containing 161 tissue spots from 152 breast cancer patients was obtained from Shanghai Outdo Biotech Co.,Ltd. The TMA was stained with antibodies against p-STAT3 Y705 (Abcam, ab76315) and HMGN5 (Invitrogen, PA5-50468). Positive signal was graded by two automatized histology quantification program (Definiens Tissue Studio® 4.0 or ZEN).
Mou J., Huang M., Wang F., Xu X., Xie H., Lu H., Li M., Li Y., Kong W., Chen J., Xiao Y., Chen Y., Wang C, & Ren J. (2022). HMGN5 Escorts Oncogenic STAT3 Signaling by Regulating the Chromatin Landscape in Breast Cancer Tumorigenesis. Molecular cancer research : MCR, 20(12).
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2

Investigating Cellular Invasion and Migration

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The cell invasion and migration assays were performed using the Transwell Permeable Supports with 8μm pore (Corning, USA, 3422). Brie y, 1.5 × 10 5 cells/cm 2 were seeded into apical chambers with the basement membrane pretreated with (for cell invasion assay) or without (for cell migration assay) 20μg Matrigel (Corning, 354248). After 24 h, the transwell was xed with 4% PFA for 15 min and stained with hematoxylin for 15 min. The cells on the upper side of basement membrane were gently removed by cotton swabs, while the cells on the lower side of basement membrane were imaged. ImageJ was then used to calculate the number of transferred cells.
Human BC TMA Tissue microarray (TMA) containing 161 tissue spots from 152 breast cancer patients was obtained from Shanghai Outdo Biotech Co.,Ltd. The TMA was stained with antibodies against p-STAT3 Y705 (Abcam, ab76315) and HMGN5 (Invitrogen, PA5-50468). Positive signal was graded by two automatized histology quanti cation program (De niens Tissue Studio® 4.0 or ZEN).
Mou J., Huang M., Wang F., Xu X., Xie H., Lu H., Li M., Li Y., Kong W., Chen J., Xiao Y., Chen Y., Wang C., & Ren J. (2022). HMGN5 escorts oncogenic STAT3 signaling by regulating chromatin landscape in tumorigenesis of breast cancer.
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