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CCl3COOH is a chemical compound used in various laboratory applications. It is a colorless, crystalline solid with a pungent odor. The core function of this product is to serve as a reagent or building block in chemical synthesis and analysis procedures. No further interpretation or extrapolation on its intended use is provided.

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3 protocols using ccl3cooh

1

Cadmium Chloride Induced Cellular Stress Response

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CA, cadmium chloride (CdCl2), fetal bovine serum (FBS), Bradford reagent, bovine serum albumin (BSA), and cell culture media were obtained from Sigma-Aldrich Chemical Company, St. Louis, USA. Antibodies for Western blotting were obtained from Cell Signaling Technology, Beverley, USA, Denver, USA, and Bio-Rad, Hercules, USA. (NH4)2SO4, 2,4-dinitrophenylhydrazine, CCl3COOH, 5-thio-2-nitrobenzoic acid, 5,5-dithiobis(2-nitrobenzoic acid), 1-chloro-2,4-dinitrobenzene, ethylene diaminetetraacetic acid (EDTA), Na4P2O7, NaN3, KH2PO4, N-ethylmaleimide, nitro blue tetrazolium (NBT), phenazinemethosulphate, reduced glutathione (GSH), reduced nicotinamide adenine dinucleotide (NADH), and thiobarbituric acid (TBA) were bought from Sisco Research Laboratory, Mumbai, India. Solvents were bought from Merck, Mumbai, India. Kits for measurement of different biochemical parameters were purchased from Span diagnostic Ltd., Kolkata, India. ELISA kits were obtained from R&D Systems, Minneapolis, USA and Thermo Fisher Scientific Co., Waltham, USA.
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2

Biochemical Analysis of Cell Cultures

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CA, bovine serum albumin (BSA), Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), Bradford reagent, and collagenase type I were procured from Sigma-Aldrich Chemical Company, MO, USA. Antibodies for immunoblotting were bought from Novus Biologicals, CO, USA. The solvents (HPLC grade) were obtained from Merck, Mumbai, India. Kits for the measurement of different biochemical parameters were procured from Span Diagnostics Ltd., India. 1-Chloro-2,4-dinitrobenzene, (NH4)2SO4, NaAsO2, 2,4-dinitrophenylhydrazine, ethylenediaminetetraacetic acid, 5,5-dithiobis(2-nitrobenzoic acid), N-Ethylmaleimide, nitro blue tetrazolium, NADH, KH2PO4, phenazine methosulphate, Na4P2O7, GSH, NaN3, thiobarbituric acid, 5-thio-2-nitrobenzoic acid, and CCl3COOH were obtained from Sisco Research Laboratory, Mumbai, India.
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3

Cadmium Chloride Cytotoxicity Assay

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Cadmium chloride (CdCl2), fetal bovine serum (FBS), Bradford reagent, APO transferrin, hydrocortisone, antibiotic antimycotic solution, and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Chemical Company, St. Louis, USA. Dulbecco’s modified Eagle’s/Ham’s F12 (DMEM-F12) and Hank’s balanced salt solution (HBSS) were procured from Thermo Fisher Scientific, USA. Antibodies for immunoblotting were obtained from Cell Signaling Technology, Beverley, USA, Denver, USA, and Bio-Rad, Hercules, USA. Furthermore, 5,5-dithiobis(2-nitrobenzoic acid), 1-chloro-2,4-dinitrobenzene, (NH4)2SO4, 2,4-dinitrophenylhydrazine, 5-thio-2-nitrobenzoic acid, CCl3COOH, ethylene diaminetetraacetic acid, Na4P2O7, NaN3, KH2PO4, N-ethylmaleimide, nitro blue tetrazolium, phenazinemethosufhate, reduced glutathione, reduced nicotinamide adenine dinucleotide (NADH), and thiobarbituric acid were purchased from Sisco Research Laboratory, Mumbai, India. HPLC-grade solvents were brought from Merck, Mumbai, India. Kits for measurement of different biochemical parameters were purchased from Span diagnostic Ltd., India.
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