HEK293T, HK-2, ACHN, 786-O, Caki-1, KETR3, and OS-RC2 cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). 786-O, Caki-1, KETR3, and OS-RC2 cells were cultured in RPMI-1640 medium (KGM31800NH-500, KeyGEN BioTECH) supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin and incubated in a 37◦C humidified incubator with 5% CO2. ACHN cells were cultured in MEM (KGM41500N-500, KeyGEN BioTECH), HEK293T, and HK-2 cells were cultured in DMEM (KGM12800NH-500, KeyGEN BioTECH), and the other cell culture conditions were the same as those described above.
Os rc2
Manufactured by Keygen Biotech
Sourced in China
The OS-RC2 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating various biological samples and materials based on their density differences. The core function of the OS-RC2 is to efficiently and reliably perform sample separation and concentration tasks in a wide range of scientific and research settings.
Automatically generated - may contain errors
Lab products found in correlation
Caki 1, by Keygen Biotech (2 mentions)
Kgm41500n 500, by Keygen Biotech (1 mentions)
Kgm12800nh 500, by Keygen Biotech (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Caki 1, by American Type Culture Collection (1 mentions)
Osrc 2, by American Type Culture Collection (1 mentions)
Ketr 3, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Keygen Biotech (1 mentions)
Dmem f12 medium, by Keygen Biotech (1 mentions)
3 protocols using os rc2
1
Culture and Maintenance of Renal Cell Lines
2
Cell Culture of Human Renal Cell Lines
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The human ccRCC cell lines (786-O, Caki-1, 769-P, OS-RC-2, and ACHN) and human renal tubular epithelial cells (HK-2) were all purchased from the Cell Resources Center, Shanghai Academy of Life Sciences, Chinese Academy of Sciences. ACHN cells were grown in RPMI-DMEM (KeyGen, Nanjing, China); HK-2, 786-O, Caki-1, 769-P, and OS-RC-2 cells were grown in RPMI 1640 medium (KeyGen, Nanjing, China) containing 10% FBS (Life Technologies, Australia). All cells were grown at 37 °C in a humidified 5% CO2 atmosphere.
3
Cell Culture of Renal Cell Lines
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Human renal tubular epithelial cell line HK-2, human renal cell carcinoma (RCC) lines 786-O, Achn, and OSRC-2 were obtained from Procell Life Science &Technology (Wuhan, China) and authenticated by the short tandem repeat (STR) method. HK-2, Achn, and OSRC-2 cells were incubated in DMEM/F12 medium (Keygen biotech, Nanjing, China), and 786-O cells were incubated in McCoy’s 5A medium (P/S) (Keygen biotech, Nanjing, China), with 10% fetal bovine serum (FBS) (Celligent, Hamilton, New Zealand) and incubated in sterile culture flasks at 37 °C with 5% CO2.
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