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Endothelial cell supplement

Manufactured by Merck Group
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Endothelial Cell Supplement is a cell culture media supplement designed to support the growth and maintenance of endothelial cells in vitro. It provides a standardized and optimized formulation of growth factors, hormones, and other essential nutrients required for the proliferation and differentiation of endothelial cells.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (2 mentions) Heracell 150i incubator, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Fibroblast growth factor (fgf), by Thermo Fisher Scientific (2 mentions) Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by GE Healthcare (2 mentions) Heparin, by Merck Group (2 mentions) Sb431542, by R&D Systems (2 mentions)

Close spelling variants of this product

Endothelial cell growth supplement (226 citations) Endothelial cell growth supplements (12 citations) Endothelial cell growth supplement from bovine pituitary (8 citations) Endothelial cell growth supplement from bovine neural tissue (5 citations) Endothelial cell growth factor supplement (4 citations)

2 protocols using endothelial cell supplement

1

Endothelial Cell Enrichment and Lentiviral Transduction

Check if the same lab product or an alternative is used in the 5 most similar protocols
exECs were generated as previously described (Seandel et al., 2008 (link)). Briefly, CD45−CD31+ cells were FACS purified and incubated with lentivirus containing either the E4ORF1 or myrAkt construct for 48 hours. Cell culture medium containing 20% FBS (SH30066.03, HyClone, GE Life Sciences), 10 mM HEPES (15630–080, Invitrogen), 1% Glutamax (35050061, Life Technologies), 1% Non-Essential Amino Acids (11140050, Life Technologies), 1% PenStrep (15240–062, Invitrogen), 50 ug/ml Heparin (H3149, Sigma), 50 ug/ml Endothelial Cell Supplement (02–102, Millipore-Sigma), 5 μM SB431542 (1614/10, R&D Systems), 20 ng/ml FGF (100–18B, Peprotech) and 10 ng/ml VEGF (450–32, Peprotech) at 37°C, 5% O2, 5% CO2 in a HERAcell 150i incubator (Thermo Fisher, USA).
Kinsella S., Evandy C.A., Cooper K., Iovino L., deRoos P.C., Hopwo K.S., Granadier D.W., Smith C.W., Rafii S, & Dudakov J.A. (2021). Attenuation of apoptotic cell detection triggers thymic regeneration after damage. Cell reports, 37(1), 109789.
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2

Endothelial Cell Enrichment and Lentiviral Transduction

Check if the same lab product or an alternative is used in the 5 most similar protocols
exECs were generated as previously described (Seandel et al., 2008 (link)). Briefly, CD45−CD31+ cells were FACS purified and incubated with lentivirus containing either the E4ORF1 or myrAkt construct for 48 hours. Cell culture medium containing 20% FBS (SH30066.03, HyClone, GE Life Sciences), 10 mM HEPES (15630–080, Invitrogen), 1% Glutamax (35050061, Life Technologies), 1% Non-Essential Amino Acids (11140050, Life Technologies), 1% PenStrep (15240–062, Invitrogen), 50 ug/ml Heparin (H3149, Sigma), 50 ug/ml Endothelial Cell Supplement (02–102, Millipore-Sigma), 5 μM SB431542 (1614/10, R&D Systems), 20 ng/ml FGF (100–18B, Peprotech) and 10 ng/ml VEGF (450–32, Peprotech) at 37°C, 5% O2, 5% CO2 in a HERAcell 150i incubator (Thermo Fisher, USA).
Kinsella S., Evandy C.A., Cooper K., Iovino L., deRoos P.C., Hopwo K.S., Granadier D.W., Smith C.W., Rafii S, & Dudakov J.A. (2021). Attenuation of apoptotic cell detection triggers thymic regeneration after damage. Cell reports, 37(1), 109789.
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