Human hepatocellular carcinoma cell lines HepG2 was purchased from ATCC and MHCC97L was gifted by Dr. Man Kwan, Department of Surgery, The University of Hong Kong. L-02 cell line was purchased from Experimental Animal Center of Sun Yat-sen University, Guangzhou. Cell lines were maintained in DMEM (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS and 1% penicillin/streptomycin. HUVEC cell line was purchased from ATCC and it was maintained in EGM-2 complete medium (Lonza, Basel, Switzerland) at 5% of CO2 incubator. The cell lines were passaged whenever they reached 80% confluency.
Huvec cell line
Manufactured by American Type Culture Collection
Sourced in United States
The HUVEC (Human Umbilical Vein Endothelial Cell) cell line is a primary cell culture derived from the vascular endothelium of human umbilical veins. This cell line is commonly used in research to study endothelial cell biology, vascular development, and angiogenesis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Complete egm 2 medium, by Lonza (1 mentions)
Huvec cells, by Corning (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Lipofectamine kit, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by Thermo Fisher Scientific (1 mentions)
Thp 1 human monocytic cell line, by American Type Culture Collection (1 mentions)
Mrs broth, by BD (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
U87 cell line, by American Type Culture Collection (1 mentions)
Protein lysis buffer, by Solarbio (1 mentions)
Lactoferrin, by Merck Group (1 mentions)
Egm 2 medium, by Thermo Fisher Scientific (1 mentions)
Elisa kit, by Nanjing Jiancheng (1 mentions)
Cell counting kit 8 cck 8, by Solarbio (1 mentions)
Arachidonic acid, by Merck Group (1 mentions)
Ox ldl, by Merck Group (1 mentions)
26 protocols using huvec cell line
1
Cell Line Maintenance Protocols
2
Prospective study of acute promyelocytic leukemia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
From May 2007 to December 2011, bone marrow and peripheral blood samples from NAPL and RAPL (hematological relapses only) admitted at our center were enrolled in this prospective study. Institutional review board approved the study design and the consent forms. Written and informed consent was obtained from all enrolled cases. (Institutional Review Board of Christian Medical College, Vellore, India: IRB, CMC, Vellore. RC Min 5884. 18th April, 2006). NAPL were treated with a single agent ATO based regimen as has been previously reported by us[11 (link), 20 (link)]. The clinical outcome in RAPL consolidated with and without an autologous SCT following induction of molecular remission has also been previously reported by us[13 (link)]. The human APL cell line NB4[21 (link)] (Kind gift from Dr Harry Iland, RPAH, Sydney, Australia with permission from Dr Michel Lanotte) was used for some experiments. HUVEC cell line (ATCC, Manassas, USA) was used as a control for stromal cells in the co-culture experiments. An ATO resistance cell line (NB4EV AsR1) was derived from the NB4 cell line in-house and was also used as a control for some experiments.
3
HUVEC Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HUVEC cell line was used for the present study and was purchased from ATCC. HUVEC cells were cultured in DMEM (Corning, Shanghai, China) with 20% (v/v) fetal calf serum (FBS, Gibco-BRL, Shanghai, China), supplemented with ECGS (MACGENE, Shanghai, China).
4
Maintaining and Transfecting HUVEC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HUVEC cell line (ATCC, Manassas, VA, USA) was maintained in Dulbecco’s modified minimal essential medium (DMEM, Life Technologies, Inc., Burlington, ON, USA) supplemented with 10% (v/v) fetal bovine serum (Life Technologies, Inc.), at 37°C, in a humidified atmosphere containing 95% air and 5% CO2. These cells were used throughout this study. For transfections, the cells were split the day prior to transfection to reach 70–80% confluence. Stable HUVEC cell transfectants expressing Cx43-NT were generated by transfection, respectively, with a Cx43-NT cDNA encoding plasmid using the Lipofectamine kit (Invitrogen Corporation, California, USA) and subsequent selection for G418 resistance according to the manufacturer’s instructions. Transfectants were tested by QPCR for Cx43-NT expression.
5
HUVEC Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HUVEC cell line was purchased from ATCC (Manassas, VA, USA). Cells were grown at 37 °C in 5 % CO2 in endothelial growth medium (EGM-2-MV) containing 2 % FBS, 12 μg/ml bovine brain extract, 10 ng/ml human recombinant epidermal growth factor, 1 μg/ml hydrocortisone, GA-1000 (gentamicin and amphotericin B, 1 μg/ml), according to the recommendations of the supplier.
6
Characterization of Probiotic Strains and Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Both LAB strains used in this study, Lactobacillus helveticus R0389 and Lactocaseibacillus rhamnosus R0011, were provided by Lallemand Health Solutions (Montreal, QC, Canada). Both strains were grown in Difco de Man, Rogosa, Sharpe (MRS) broth (BD Diagnostic Systems, Sparks, MD) at 37°C under agitation (200-220 rpm) , unless otherwise stated.
The THP-1 human monocytic cell line (ATCC #TIB-202) was used for all immunological studies. The THP-1 cells were grown in RPMI 1640 growth medium (supplemented with 10% fetal bovine serum, 0.05 mg/ mL gentamicin, and 0.05 mM β-mercaptoethanol) at 37°C with 5% CO 2 . Cells were subcultured every 3 to 4 d.
The HUVEC cell line (ATCC #CRL-1730) was used for all NO liberation studies. The HUVEC were grown in MCBD-131 growth medium (supplemented with 10% fetal bovine serum, 1 mg/mL epidermal growth factor, 50 μg/mL hydrocortisone, 10 mM l-glutamine, 10,000 units/mL penicillin, and 10 mg/mL streptomycin) at 37°C with 5% CO 2 . Cells were subcultured at 80% confluence and were used at passage 2 to perform all assays.
The THP-1 human monocytic cell line (ATCC #TIB-202) was used for all immunological studies. The THP-1 cells were grown in RPMI 1640 growth medium (supplemented with 10% fetal bovine serum, 0.05 mg/ mL gentamicin, and 0.05 mM β-mercaptoethanol) at 37°C with 5% CO 2 . Cells were subcultured every 3 to 4 d.
The HUVEC cell line (ATCC #CRL-1730) was used for all NO liberation studies. The HUVEC were grown in MCBD-131 growth medium (supplemented with 10% fetal bovine serum, 1 mg/mL epidermal growth factor, 50 μg/mL hydrocortisone, 10 mM l-glutamine, 10,000 units/mL penicillin, and 10 mg/mL streptomycin) at 37°C with 5% CO 2 . Cells were subcultured at 80% confluence and were used at passage 2 to perform all assays.
7
Culturing Human Glioma and Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human glioma U87 cell line was purchased from the American Type Culture Collection (ATCC, USA) and cultured in Eagle’s Minimum Essential Medium with 1.5 g L− 1 sodium bicarbonate, nonessential amino acids, L-glutamine, sodium pyruvate, and 1% Penicillin/Streptomycin (Corning, NY, USA) with 10% heat-inactivated fetal bovine serum (FBS, GWVITEK, Seoul, Korea). The human umbilical vein endothelial cell (HUVEC) cell line was purchased from ATCC and cultured in EGM2 medium with supplements. Confluent cells were passaged every 4–5 days using 0.05% trypsin/ethylenediaminetetraacetic acid. All the cells were maintained in an incubator at 37 °C with 5% CO2 in a humidified atmosphere.
8
Comprehensive Cancer Cell Line Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human lung cancer cell lines A549, NCI-H1975, Hcc827, human colon cancer lines HCT 116 and HT-29, human breast cancer cell lines MDB-MA-231 and MCF-7, human gastric cancer cell lines MGC80-3 and MKN-28, human cervical cancer cell lines HeLa and SiHaand Caski, human ovarian cancer cell line HO-8910, human hepatocacinoma cell line BEL-7402, human prostate cancer cell line PC-3, human leukemia cell lines K562 and HL-60, and human normal liver cell line L-02 were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). The HUVEC cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were cultured in DMEM (HCT 116, HT-29), RPMI 1640 (NCI-H1975, Hcc827, MGC80-3, MKN-28, HO-8910, BEL-7402, PC-3, K562, HL-60, L-02, HUVEC), F12K(A549), and MEM (MCF-7, HeLa, SiHa, Caski,) medium, containing 100 U/mL penicillin, 100 mg/mL streptomycin, and 10% fetal bovine serum, in a humidified atmosphere of 5% CO2 at 37.0 °C.
9
Lactoferrin Modulates HUVEC Angiogenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactoferrin, of 95% purity, was purchased from Sigma (St. Louis, MO, USA). The HUVEC cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). EGM-2 medium and fetal bovine serum (FBS) were obtained from Gibco (Waltham, MA, USA); 1% streptomycin/penicillin was purchased from Thermo Fisher (Waltham, MA, USA). Cell Counting Kit-8 (CCK8) was purchased from Solarbio (Beijing, China). ELISA kits were purchased from Jiancheng (Nanjing, China). The PDXP siRNA fragment was synthesized by Sangon (Shanghai, China). The primary and secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Protein lysis buffer and the related reagents were purchased from Solarbio. Enhanced chemiluminescence (ECL) reagent was purchased from Tanon (Shanghai, China).
10
Molecular Modeling of Phytophthora ramorum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Δ-17 FAD: The original sequence of Phytophthora ramorum was obtained from NCBI: FW362214.1;
HUVEC cell line (American Type Culture Collection, Manassas, Virginia);
Lentiviral vector: pLV[Exp]-Neo (Cyagen);
Arachidonic acid and ox-LDL (Sigma-Aldrich, St. Louis, MO);
VEGF antibody (R&D Systems, Minneapolis, MN).
HUVEC cell line (American Type Culture Collection, Manassas, Virginia);
Lentiviral vector: pLV[Exp]-Neo (Cyagen);
Arachidonic acid and ox-LDL (Sigma-Aldrich, St. Louis, MO);
VEGF antibody (R&D Systems, Minneapolis, MN).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!