The human glioblastoma cell lines A172, U-118MG, and U-87MG were purchased from the cell bank of the Chinese Academy of Sciences in Shanghai, and SF-268 was purchased from American Tissue Culture Collection. U373 and U251 were obtained as gifts from Prof. Yiping Li (Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University North Campus). All cell lines were maintained in DMEM supplemented with 10% FBS, 100 μg/ml penicillin, and 100 μg/ml streptomycin, except SF-268, which was maintained in RPMI 1640 medium. Cells were incubated in a humidified atmosphere containing 5% CO2 at 37 °C.
U118mg
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The U118MG is a cell line derived from a human glioblastoma, a type of brain tumor. It is commonly used in research and development for the study of brain cancer and related neurological conditions. The core function of this cell line is to provide a standardized and authenticated model for in vitro experiments and analyses.
Automatically generated - may contain errors
Lab products found in correlation
U251mg, by National Collection of Authenticated Cell Cultures (6 mentions)
U87mg, by National Collection of Authenticated Cell Cultures (5 mentions)
Penicillin, by National Collection of Authenticated Cell Cultures (2 mentions)
Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Scrambled sirna, by Santa Cruz Biotechnology (1 mentions)
Streptomycin, by National Collection of Authenticated Cell Cultures (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Recombinant human bfgf, by R&D Systems (1 mentions)
Recombinant human egf, by R&D Systems (1 mentions)
Ln229, by National Collection of Authenticated Cell Cultures (1 mentions)
Accutase solution, by Merck Group (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
B 27 no serum supplement, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Ln229, by American Type Culture Collection (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
8 protocols using u118mg
1
Cell Culture of Human Glioblastoma Lines
2
In Vitro Glioma Cell Culturing
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Human glioma cell line U251MG and human glioblastoma cell lines U118MG and U87MG were all purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM containing 10% fetal bovine serum (FBS) supplemented with streptomycin (100 μg/ml) and penicillin (100 U/ml). The antibodies without special instructions were all purchased as follows: anti-NRP2 (sc-13117, Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-VEGF-C (AP2042d, ABGENT, San Diego, CA, USA) ; and anti-βactin (sc-47778, Santa Cruz Biotechnology, Santa Cruz, CA, USA) .
3
Silencing PTPN3 in Glioma Cell Lines
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Human GBM cell lines U118 MG and A172 and glioma cell line U251 MG were all purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM containing 10% fetal bovine serum (FBS) supplemented with streptomycin (100 μg/ml) and penicillin (100 U/ml). The siRNA of PTPN3 and the scrambled siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Transfection was performed using Lipofectamine 2000 according to the manual.
4
Glioblastoma Cell Line Cultivation and Antibody Usage
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Human glioma cell line U251MG, human glioblastoma cell lines U118MG and U87MG, A172 were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, People’s Republic of China). All these cells were cultured in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum supplemented with streptomycin (100 μg/mL) and penicillin (100 U/mL). MECOM primary antibody (Cat. 28100002) was from Novus Biologicals (Littleton, CO, USA). Lamin-A antibody (sc-6214) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
5
GBM Cell Lines Characterization
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Human GBM cell lines U251 MG, U118 MG, and A172 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM containing 10% fetal bovine serum (FBS) supplemented with streptomycin (100 μg/ml) and penicillin (100 U/ml). All reagents were purchased from Sigma-Aldrich Company unless specified otherwise. Applied antibodies were: primary antibodies of ERK, AKT, phosphorylated ERK (Tyr202/204), AKT (Ser473) (CellSignaling Technology, Danvers, MA, USA), and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Human recombinant HMGB1 was from Sino Biotech (Beijing, China).
6
Cultivating Human Glioma Cells
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Normal human astrocytes (NHAs) and human glioma cell lines A172, T98G, U118MG, and U251 were purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). The cells were cultured in the medium of RPMI 1640 (Hyclone) and 10% FBS (Gibco) in a humidified incubator at 37°C with 5%CO2. When the degree of cell fusion reached more than 80%, the cells were obtained and used for subsequent experiments.
7
Glioblastoma Cell Lines and Glioma Stem Cells
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Human glioma cell lines U118MG, U87MG, LN229 and U251MG were purchased from the Chinese Academy of Sciences Cell Bank and cultured in DMEM medium (Gibco, USA) with 10% fetal bovine serum (FBS). The neural progenitor cell (NPC) and GBM patient-derived GSC cell lines and were kindly donated by Dr. Krishna P.L. Bhat (The University of Texas, M.D. Anderson Cancer Center, Houston, TX). GSC lines (GSC20, GSC267, GSC8–11, GSC11) have been used extensively in previous studies and the subtypes of GSCs have been clarified according to the Verhaak or Philips gene signatures, respectively. GSCs and NPC were cultured in DMEM/F12 (Gibco, USA) with 2% B-27 no serum supplement (Gibco, USA), 20 ng/mL human recombinant bFGF (R&D Systems) as well as 20 ng/mL human recombinant EGF (R&D Systems, USA). The GSC or NPC spheres were digested using accutase solution (Sigma-Aldrich, USA). All cell lines were cultured in a humid chamber at 37 °C and containing 5% carbon dioxide and 5% oxygen.
8
Glioma Cell Line Cultivation Protocol
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U118MG, U87MG, and U251MG cells were purchased from the Chinese Academy of Sciences Cell Bank (China) and LN229, LN18, A172, and T98G cell lines were obtained from ATCC (USA). TJ905 cell was separated from human GBM tissue and was cultivated in a mixture consisting of 10% fetal bovine serum (FBS, Gibco, USA) and DMEM/F12 medium (Gibco, USA). DMEM (Gibco, USA) mixed with 10% FBS was used to cultured other seven glioma cell lines which were incubated in 5% CO 2 at constant temperature of 37 °C. Cells were identified by polymorphic short tandem repeat profiling in advance and tested every 6 months for excluding mycoplasma contamination.
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